Linked Life Data

14273661

Source:http://linkedlifedata.com/resource/pubmed/id/14273661

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1965-7-1
pubmed:abstractText
Reilly, Bernard E. (Western Reserve University, Cleveland, Ohio), and John Spizizen. Bacteriophage deoxyribonucleate infection of competent Bacillus subtilis. J. Bacteriol. 89:782-790. 1964.-Phenol extracts of the Bacillus subtilis bacteriophages phi1, phi25, and phi29 contained infectious deoxyribonucleic acid. The infectivity was destroyed by catalytic amounts of deoxyribonuclease but not by specific phage antiserum, ribonuclease, or trypsin. An infectivity of >10(6) infectious centers formed per mug of deoxyribonucleic acid (DNA) added was obtained. The stability of the infectious centers permitted an examination of a single cycle of phage replication in cells unable to adsorb the mature virus. A typical cycle was observed, although the latent period was increased and the burst size slightly reduced after DNA infection. The development of competence for bacterial transformation was strongly correlated with susceptibility to viral DNA infection. Both appeared and disappeared at the same phase of growth in the cell population. More than 4% of the viable cells in the competent population were infected by viral DNA. The kinetics of the transition of phi29 DNA infection to deoxyribonuclease insensitivity, and the relationship of infectivity to DNA dilution, were similar to the results obtained for bacterial transformation of a single marker. The doseresponse curve of phi1 and phi25 DNA was characteristic of that obtained in multiple transformation of unlinked genetic markers. Because of the low efficiency of infection, about 10(-4) per phage equivalent of DNA added, it was not possible to prove that DNA alone was sufficient to initiate infection.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-12981234, http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-13750787, http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-13787458, http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-13900279, http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-14081936, http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-14123302, http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-14196020, http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-14420563, http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-14453145, http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-14493621, http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-16561900, http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-16590070, http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-16590118, http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-16590310, http://linkedlifedata.com/resource/pubmed/commentcorrection/14273661-16590544
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
782-90
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1965
pubmed:articleTitle
BACTERIOPHAGE DEOXYRIBONUCLEATE INFECTION OF COMPETENT BACILLUS SUBTILIS.
pubmed:publicationType
Journal Article