Linked Life Data

14252292

Source:http://linkedlifedata.com/resource/pubmed/id/14252292

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1965-5-1
pubmed:abstractText
Clean microscopic slides are dipped in a solution of mercuric chloride and sucrose in 30% alcohol and allowed to dry. A thin transparent film of cytological fixative is thus formed on both sides of the slide. Fixative slides, prepared in this manner, can be stored indefinitely provided that they are not exposed to a warm, humid atmosphere. When a drop of fluid containing bacteria, protozoa, or mammalian cells is spread over the surface of such a slide the formed elements are immediately fixed, the finer cytological details being well preserved. Staining and examination are carried out when the specimen arrives at the laboratory. This method is particularly suitable for a diagnostic laboratory service in which most of the specimens arrive by mail. The method has been successfully employed in the diagnosis of Trichomonas vaginalis infection of the female genital tract. This parasite was found by phase-contrast microscopy in 47% of 1,000 women examined at a police court clinic. Fixative slide preparations were also obtained from these women at the time of their medical examination. Of these specimens, 38.6% showed the presence of Tr. vaginalis, and 10% were also found to be infected with N. gonorrhoeae. The fixative slide technique may also prove of value in such widely separated fields as exfoliative cytology and the diagnosis of primary syphilis.
pubmed:commentsCorrections
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9746
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27-30
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1965
pubmed:articleTitle
PREPARATION AND USE OF FIXATIVE SLIDES FOR THE DIAGNOSIS OF TRICHOMONAS VAGINALIS.
pubmed:publicationType
Journal Article