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rdf:type |
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lifeskim:mentions |
umls-concept:C0004561,
umls-concept:C0009013,
umls-concept:C0019721,
umls-concept:C0020852,
umls-concept:C0024141,
umls-concept:C0030705,
umls-concept:C0039194,
umls-concept:C0178587,
umls-concept:C0205263,
umls-concept:C0282056,
umls-concept:C1533691
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pubmed:issue |
2
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pubmed:dateCreated |
1992-12-10
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pubmed:abstractText |
An HLA-DR restricted T cell clone (26G11) which recognized a lymphoid cell-derived autoantigen associated with DR4 molecule was shown to induce not only autologous but also allogenic DR4+ B cells to produce large amounts of antibodies of the IgG and IgM classes. Using the helper activity of this clone, we investigated the mechanism of anti-DNA antibody production in DR-matched patients with systemic lupus erythematosus (SLE). When cultured with 26G11 cells, B cells from DR-matched normal control subjects produced large amounts of IgM anti-DNA antibody, but did not produce IgG anti-DNA antibody which is thought to have a pathological role in SLE. In contrast, B cells from DR-matched patients with active SLE spontaneously produced a fairly large amount of IgG anti-DNA antibody, and the production was augmented by the T cell clone. Little IgG anti-DNA antibody was produced by the B cells of patients with inactive SLE in either the presence or absence of T cell clone. We next fractionated B cells into low density B (LD-B) and high density B (HD-B) cells by centrifugation on discontinuous Percoll density gradients. IgG anti-DNA antibody was spontaneously produced by LD-B cells of active SLE patients but not by those either of inactive SLE patients or normal controls. On the other hand, although IgG anti-DNA antibody was not spontaneously produced by the HD-B cells of both active and inactive SLE patients, it could easily be induced by their culture with the T cell clone. Our results clearly show the existence of IgG anti-DNA antibody-producing B cells in the peripheral blood of SLE patients irrespective of their disease activity and suggest that autoreactive T cells may play a pathogenic role in SLE through the induction of autoantibody production.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-1079727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-1622891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-1699879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-2144899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-2146209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-2150836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-2525144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-2826542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-2952711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-2952749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-2965184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-299748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-311204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-3876099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-3917279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-3919141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-3932473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-4132884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-4866347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-6268340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-6355300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-6399976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-6447163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1424281-7138600
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
245-50
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1424281-Antibodies, Antinuclear,
pubmed-meshheading:1424281-B-Lymphocytes,
pubmed-meshheading:1424281-HLA-DR4 Antigen,
pubmed-meshheading:1424281-Humans,
pubmed-meshheading:1424281-Immunoglobulin G,
pubmed-meshheading:1424281-Lupus Erythematosus, Systemic,
pubmed-meshheading:1424281-Lymphocyte Activation,
pubmed-meshheading:1424281-Lymphocyte Cooperation,
pubmed-meshheading:1424281-T-Lymphocytes,
pubmed-meshheading:1424281-T-Lymphocytes, Helper-Inducer
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pubmed:year |
1992
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pubmed:articleTitle |
In vitro induction of IgG anti-DNA antibody from high density B cells of systemic lupus erythematosus patients by an HLA DR-restricted T cell clone.
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pubmed:affiliation |
Second Division of Internal Medicine, School of Medicine, Kyoto University, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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