Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1965-3-1
pubmed:abstractText
Rat liver mitochondria allowed to accumulate maximal amounts of Ca(++) and HPO(4) (=) ions from the suspending medium in vitro during respiration have a considerably higher specific gravity than normal mitochondria and may be easily separated from the latter by isopycnic centrifugation in density gradients of sucrose or cesium chloride. When the mitochondria are allowed to accumulate less than maximal amounts of Ca(++) and HPO(4) (=) from the medium, they have intermediate specific gravities which are roughly proportional to their content of calcium phosphate. Maximally "loaded" mitochondria are relatively homogeneous with respect to specific gravity. Correlated biochemical and electron microscopic studies show that Ca(++)-loaded mitochondria contain numerous dense granules, of which some 85 per cent are over 500 A in diameter. These granules are electron-opaque not only following fixation and staining with heavy metal reagents, but also following fixation with formaldehyde, demonstrating that the characteristic granules in Ca(++)-loaded mitochondria have intrinsic electron-opacity. The dense granules are almost always located within the inner compartment of the mitochondria and not in the space between the inner and outer membranes. They are frequently located at or near the cristae and they often show electron-transparent "cores." Such granules appear to be made up of clusters of smaller dense particles, but preliminary x-ray diffraction analysis and electron diffraction studies have revealed no evidence of crystallinity in the deposits. The electron-opaque granules decrease in number when the Ca(++)-loaded mitochondria are incubated with 2,4-dinitrophenol; simultaneously there is discharge of Ca(++) and phosphate from the mitochondria into the medium.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-13271690, http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-13428781, http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-13706895, http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-13764136, http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-13885269, http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-13925019, http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-13986421, http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-13992515, http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-14015424, http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-14015427, http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-14085406, http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-14087335, http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-14105220, http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-14454024, http://linkedlifedata.com/resource/pubmed/commentcorrection/14228516-14479155
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
21-38
pubmed:dateRevised
2011-9-28
pubmed:meshHeading
pubmed:year
1964
pubmed:articleTitle
EFFECT OF ACTIVE ACCUMULATION OF CALCIUM AND PHOSPHATE IONS ON THE STRUCTURE OF RAT LIVER MITOCHONDRIA.
pubmed:publicationType
Journal Article