Linked Life Data

1422179

Source:http://linkedlifedata.com/resource/pubmed/id/1422179

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-12-9
pubmed:abstractText
Brush border membrane vesicles (BBMV) isolated from the rabbit renal external cortex were used to investigate inorganic cadmium (Cd) uptake. BBMV were incubated during 1 to 60 min in a buffered solution containing 10 to 200 mumol/L Cd (CdCl2), and uptake was determined using a rapid filtration technique. Cd uptake by BBMV was time-and concentration-dependent. At the lowest concentration (10 mumol/L), Cd uptake was nearly complete (70% of the plateau value, which was obtained after 10 min) within 1 min. At the highest concentration (200 mumol/L), the percentage of Cd uptake during the first minute was smaller (50%) and a slower component of uptake appeared. From additional experiments where Cd-loaded BBMV were submitted to an osmotic shock or to variations of extravesicular osmolality, it was concluded that the uptake of Cd by BBMV resulted essentially from membrane binding and not from intravesicular accumulation. The change in pH from 7.4 to 5.8 under iso- or hypotonic conditions removed about 40% of Cd bound to the BBMV, indicating that Cd binding to the membrane is pH sensitive. Thus, binding to the brush border membrane constitutes an initial step for inorganic Cd entry into renal epithelial cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0931-2838
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
111-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Cadmium uptake by brush border membrane vesicles from the rabbit renal external cortex.
pubmed:affiliation
Institut de Pharmacologie et de Toxicologie, Université de Lausanne, Switzerland.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't