|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0008565,
umls-concept:C0014162,
umls-concept:C0086300,
umls-concept:C0181904,
umls-concept:C0441655,
umls-concept:C0596972,
umls-concept:C0728873,
umls-concept:C1510438,
umls-concept:C1521743,
umls-concept:C1524063,
umls-concept:C1704646
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|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1992-12-16
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|
pubmed:abstractText |
Peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase F (PNGase F) and endo-beta-N-acetyl glucosaminidase F (Endo F) activities were monitored during cultivation of Flavobacterium meningosepticum using a new fluorescence-HPLC procedure based on a commercially available substrate. The PNGase F activity reached a maximum level at the end of the log phase and remained constant during the stationary phase, while Endo F continuously increased until late stationary phase. PNGase F obtained at the end of the log phase was less contaminated by other proteins compared with late stationary phase.
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|
pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0282-0080
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|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
9
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
162-7
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|
pubmed:dateRevised |
2006-11-15
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|
pubmed:meshHeading |
|
|
pubmed:year |
1992
|
|
pubmed:articleTitle |
Use of resorufin-labelled N-glycopeptide in a high-performance liquid chromatography assay to monitor endoglycosidase activities during cultivation of Flavobacterium meningosepticum.
|
|
pubmed:affiliation |
Institute de Biotechnologie, Université de Limoges, France.
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|
pubmed:publicationType |
Journal Article
|
