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In this paper an analysis of genetic recombinants derived from crosses between two mutant stocks of Vibrio cholerae, strain 162, differing from one another in nutritional requirements and other characters, is presented. It is shown that parent strains possessing a fertility factor (designated as the P factor) function as gene-donors while P(-) strains (without the P factor) serve as gene-recipients. Linkage between two genetic factors (purine and valine + isoleucine) is demonstrated and the probable sequence of seven genetic factors in the chromosome of V. cholerae, strain 162, is inferred.
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