Linked Life Data

1421400

Source:http://linkedlifedata.com/resource/pubmed/id/1421400

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1992-12-7
pubmed:databankReference
pubmed:abstractText
Chronic lymphocytic leukemia of B-cell origin (B-CLL) is generally thought to arise by neoplastic transformation of B lymphocytes, which express CD5 and have features of an early stage of B-cell differentiation. To study isotype-switched B-CLL as a potentially more differentiated variant, we performed genetic and functional immunoglobulin (Ig) gene analysis in two cases of CD5+ B-CLL in which the peripheral blood mononuclear cells (PBMC) secreted predominantly IgA (CLL-249) or IgG (CLL-412) when stimulated with pokeweed mitogen in vitro. By cDNA sequencing and by studies of CLL-heterohybridomas, CLL-249 expresses the heavy chain constant region C alpha as anticipated, while CLL-412 expresses C mu, not C gamma. In CLL-249, the expressed VH gene is 98% homologous to VH26, a germline VH3 gene that occurs frequently in the fetal repertoire, and which has been associated with anti-DNA specificity. The VL gene of CLL-249 is a lambda VL gene for which the germline sequence is not known. In CLL-412, the VH gene is 100% homologous to the VH1 gene of a published anti-DNA antibody (21/28), and is probably a germline gene sequence; the VL gene is 100% homologous to 15AVKI, also a germline gene. The supernatant antibody of the CLL-412 heterohybridoma is an IgM-kappa, which reacts with ssDNA and cardiolipin. The CLL-249 heterohybridoma secreted IgA-lambda, which bound none of the antigens tested, a finding that may be related to amino acid differences from the probable germline V genes. The demonstration of an in vivo isotype-switched variant, such as CLL-249, suggests that B-CLL may be a heterogeneous group of clonal disorders, of which less common variants may have features of more differentiated B-cell stages, such as isotype switching.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2287-97
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:1421400-Amino Acid Sequence, pubmed-meshheading:1421400-Base Sequence, pubmed-meshheading:1421400-Blotting, Southern, pubmed-meshheading:1421400-Cardiolipins, pubmed-meshheading:1421400-Cloning, Molecular, pubmed-meshheading:1421400-DNA, Neoplasm, pubmed-meshheading:1421400-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:1421400-Genes, Immunoglobulin, pubmed-meshheading:1421400-Genetic Variation, pubmed-meshheading:1421400-Humans, pubmed-meshheading:1421400-Hybridomas, pubmed-meshheading:1421400-Immunoglobulin A, pubmed-meshheading:1421400-Immunoglobulin Heavy Chains, pubmed-meshheading:1421400-Immunoglobulin Variable Region, pubmed-meshheading:1421400-Leukemia, Lymphocytic, Chronic, B-Cell, pubmed-meshheading:1421400-Molecular Sequence Data, pubmed-meshheading:1421400-Monocytes, pubmed-meshheading:1421400-Oligodeoxyribonucleotides
pubmed:year
1992
pubmed:articleTitle
Variable region gene analysis of an isotype-switched (IgA) variant of chronic lymphocytic leukemia.
pubmed:affiliation
Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia 19104.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't