Linked Life Data

14196818

Source:http://linkedlifedata.com/resource/pubmed/id/14196818

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1965-1-1
pubmed:abstractText
In the investigation of outbreaks of plague it is frequently more satisfactory to send specimens of suspected material collected in the field to a central laboratory, where usually better facilities for their more detailed examination are available. In the present study the authors have investigated the suitability of solid CO(2) (dry-ice) for the preservation of such material during transit. Three types of preparation were tested: broth suspensions of Pasteurella pestis, the livers and spleens of guinea-pigs dying after being infected with two different strains of P. pestis, and whole carcasses of mice and ground-squirrels infected with the organism. An additional test to ascertain the rate at which animal specimens became frozen and thawed was also carried out.These studies showed that (1) organisms in the various tissues frozen in dry-ice were not adversely affected by such treatment; (2) the survival of P. pestis cells did not depend on the number of organisms present in broth cultures or tissue suspensions, small numbers surviving equally well as large; and (3) plague bacilli contained in whole carcasses, even when present in small numbers, were also successfully preserved. It is concluded from these results, and also from the authors' practical use of the method over several years, that for the transport of plague-suspect materials from the field to the laboratory freezing with dry-ice can be confidently recommended.
pubmed:commentsCorrections
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0042-9686
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
741-6
pubmed:dateRevised
2010-10-26
pubmed:meshHeading
pubmed:year
1964
pubmed:articleTitle
THE SURVIVAL OF PASTEURELLA PESTIS IN MATERIALS PRESERVED BY SOLID CARBON DIOXIDE (DRY-ICE).
pubmed:publicationType
Journal Article