1417783

umls-concept:C0031669, umls-concept:C0031670, umls-concept:C0033634, umls-concept:C0035820, umls-concept:C0043791, umls-concept:C0178797, umls-concept:C0220781, umls-concept:C0225336, umls-concept:C0332120, umls-concept:C0871261, umls-concept:C1314939, umls-concept:C1704632, umls-concept:C1706817, umls-concept:C1879547, umls-concept:C1883254, umls-concept:C1948023, umls-concept:C2911692

To investigate the stimulation of phosphatidic acid formation in bovine aortic endothelial cells by P2-purinergic agonists, we labelled AG4762 cells with [32P]P1 and stimulated in the presence of butanol. Under these conditions phospholipase D generated [32P]phosphatidylbutanol, whereas the [32P]phosphatidic acid from phospholipase C and diacylglycerol kinase was unchanged. The action of various purinergic agonists on both [32P]phosphatidic acid and [32P]phosphatidylbutanol was consistent with the presence of a P2Y receptor. The stimulation of phospholipase D was dependent on extracellular Ca2+ and was mostly transient (completed within 3 min), whereas the initial stimulation of phospholipase C was independent of extracellular Ca2+, followed by a Ca(2+)-dependent phase. The agonist stimulation of phospholipase D was dependent on protein kinase C, as judged by its sensitivity to the relatively selective protein kinase C inhibitor Ro 31-8220. These results show that purinergic-receptor-mediated stimulation of phosphatidic acid has three phases: an initial Ca(2+)-independent stimulation of phospholipase C, an early but transient Ca(2+)- and protein kinase C-dependent stimulation of phospholipase D, and a sustained Ca(2+)-dependent stimulation of phospholipase C. Using propranolol to inhibit phosphatidate phosphohydrolase, we provide evidence that phosphatidic acid derived from purinergic-receptor-mediated stimulation of the phospholipase C/diacylglycerol kinase route can itself be converted back into diacylglycerol.

http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-1545777, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-1713614, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-1747119, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-1861147, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-1998501, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2002344, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2035689, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2104616, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2107183, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2164632, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2176212, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2201284, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2390085, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2394701, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2408210, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2508628, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2515851, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2532156, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2557846, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2722803, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2844277, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2985402, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-2996968, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-3064854, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-3098574, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-3291862, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-3500950, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-3533627, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-3572809, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-4425092, http://linkedlifedata.com/resource/pubmed/commentcorrection/1417783-6022844

http://linkedlifedata.com/resource/pubmed/journal/2984726R

http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Diglycerides, http://linkedlifedata.com/resource/pubmed/chemical/Indoles, http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidic Acids, http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase D, http://linkedlifedata.com/resource/pubmed/chemical/Propranolol, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic, http://linkedlifedata.com/resource/pubmed/chemical/Ro 31-8220, http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases

Oct

pubmed-author:BoarderM RMR, pubmed-author:PurkissJ RJR

1

NLM

31-6

pubmed-meshheading:1417783-Adenosine Triphosphate, pubmed-meshheading:1417783-Animals, pubmed-meshheading:1417783-Calcium, pubmed-meshheading:1417783-Cattle, pubmed-meshheading:1417783-Cells, Cultured, pubmed-meshheading:1417783-Diglycerides, pubmed-meshheading:1417783-Endothelium, Vascular, pubmed-meshheading:1417783-Indoles, pubmed-meshheading:1417783-Phosphatidic Acids, pubmed-meshheading:1417783-Phospholipase D, pubmed-meshheading:1417783-Propranolol, pubmed-meshheading:1417783-Protein Kinase C, pubmed-meshheading:1417783-Receptors, Purinergic, pubmed-meshheading:1417783-Signal Transduction, pubmed-meshheading:1417783-Type C Phospholipases

Stimulation of phosphatidate synthesis in endothelial cells in response to P2-receptor activation. Evidence for phospholipase C and phospholipase D involvement, phosphatidate and diacylglycerol interconversion and the role of protein kinase C.

Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't