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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1992-11-10
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pubmed:abstractText |
A fragment of the genome of human immunodeficiency virus type 1 (HIV-1) coding for p23 protein, the product of vif gene, was cloned in a plasmid vector pUR291. The resulting recombinant plasmid pLacVif1 was conducive in E. coli cells to the synthesis of a hybrid polypeptide with molecular weight of 136 kDa containing antigenic determinants of p23 protein of HIV-1. The employment of this polypeptide for analysis of HIV-1-positive sera by indirect enzyme immunoassay showed that vif-specific antibodies were found in 53% of the cases and their appearance was not related to the stage of the disease.
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pubmed:language |
rus
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0507-4088
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
16-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1413707-Antibody Specificity,
pubmed-meshheading:1413707-Cloning, Molecular,
pubmed-meshheading:1413707-Escherichia coli,
pubmed-meshheading:1413707-Gene Products, vif,
pubmed-meshheading:1413707-HIV Antibodies,
pubmed-meshheading:1413707-HIV Seropositivity,
pubmed-meshheading:1413707-HIV-1,
pubmed-meshheading:1413707-Humans,
pubmed-meshheading:1413707-Immunoenzyme Techniques,
pubmed-meshheading:1413707-Molecular Weight,
pubmed-meshheading:1413707-Plasmids,
pubmed-meshheading:1413707-Recombinant Proteins,
pubmed-meshheading:1413707-vif Gene Products, Human Immunodeficiency Virus
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pubmed:articleTitle |
[The use of the vif gene expression product of HIV-1 in bacteria for detecting specific antibodies in the sera of infected persons].
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pubmed:publicationType |
Journal Article,
English Abstract,
Research Support, Non-U.S. Gov't
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