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pubmed:abstractText |
Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F' incorporating them. J. Bacteriol. 87:554-561. 1964.-The gene responsible for thymine requirement or independence in Escherichia coli K-12 is located on the chromosome, near Arg(2) (arginine) and between Sm (streptomycin) and Ade (adenine). A new F' (called F(15)) carrying the Thy (thymine) gene as well as the Arg(2) gene was discovered. The Thy(+) (thymine independent) gene on F(15) relieves the defect of all Thy(-) (thymine requiring) mutants of E. coli examined, such as E. coli 15 T(-), B3, and Thy(-) mutants obtained by aminopterine treatment. It can also be transferred to Thy(-) mutants of Salmonella typhimurium and Serratia marcescens, converting them to Thy(+).
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