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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5080
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pubmed:dateCreated |
1992-11-12
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pubmed:abstractText |
The development of high brightness and short pulse width (< 200 picoseconds) x-ray lasers now offers biologists the possibility of high-resolution imaging of specimens in an aqueous environment without the blurring effects associated with natural motions and chemical erosion. As a step toward developing the capabilities of this type of x-ray microscopy, a tantalum x-ray laser at 44.83 angstrom wavelength was used together with an x-ray zone plate lens to image both unlabeled and selectively gold-labeled dried rat sperm nuclei. The observed images show approximately 500 angstrom features, illustrate the importance of x-ray microscopy in determining chemical composition, and provide information about the uniformity of sperm chromatin organization and the extent of sperm chromatin hydration.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0036-8075
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
258
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
269-71
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pubmed:dateRevised |
2007-3-19
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pubmed:meshHeading |
pubmed-meshheading:1411525-Animals,
pubmed-meshheading:1411525-Cell Fractionation,
pubmed-meshheading:1411525-Cell Nucleus,
pubmed-meshheading:1411525-Chromatin,
pubmed-meshheading:1411525-DNA,
pubmed-meshheading:1411525-Epididymis,
pubmed-meshheading:1411525-Immunohistochemistry,
pubmed-meshheading:1411525-Lasers,
pubmed-meshheading:1411525-Male,
pubmed-meshheading:1411525-Microscopy,
pubmed-meshheading:1411525-Rats,
pubmed-meshheading:1411525-Spermatozoa,
pubmed-meshheading:1411525-X-Rays
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pubmed:year |
1992
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pubmed:articleTitle |
X-ray laser microscopy of rat sperm nuclei.
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pubmed:affiliation |
Department of Physics, University of California, Berkeley 94720.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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