Linked Life Data

14112262

Source:http://linkedlifedata.com/resource/pubmed/id/14112262

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1964-6-1
pubmed:abstractText
The influence of phagocytosis on the morphological and biochemical properties of macrophage hydrolase-containing granules has been studied in vitro. Following the uptake of large numbers of heat-killed bacteria, an intracellular rearrangement of hydrolytic enzymes occurred. This was associated with the solubilization of 50 to 60 per cent of the total cell content of acid phosphatase, cathepsin, lysozyme, beta glucuronidase, acid ribonuclease, and acid desoxyribonuclease and with a corresponding decrease in granule-bound enzyme. With more prolonged incubation the majority of the soluble intracellular pool of acid ribonuclease and lysozyme was lost to the extracellular medium. No change in the total content of any of the hydrolases was noted during 180 minutes of incubation in vitro. The morphological fate of the granules was studied by a histochemical method for acid phosphatase. After the phagocytosis of yeast cell walls there was a disappearance of acid phosphatase-positive granules and an accumulation of reaction product about the ingested particle. Experiments employing macrophages which were supravitally stained with neutral red also demonstrated the loss of neutral red-positive granules and the accumulation of the dye about the yeast cell walls. These results strongly suggest that lysis of macrophage granules occurs following phagocytosis and that a portion of the granule contents are then resegregated within the newly formed phagocytic vacuole.
pubmed:commentsCorrections
pubmed:keyword
http://linkedlifedata.com/resource/pubmed/keyword/ACID PHOSPHATASE, http://linkedlifedata.com/resource/pubmed/keyword/BIOCHEMISTRY, http://linkedlifedata.com/resource/pubmed/keyword/CATHEPSIN, http://linkedlifedata.com/resource/pubmed/keyword/ESCHERICHIA COLI, http://linkedlifedata.com/resource/pubmed/keyword/EXPERIMENTAL LAB STUDY, http://linkedlifedata.com/resource/pubmed/keyword/LEUKOCYTES, http://linkedlifedata.com/resource/pubmed/keyword/MACROPHAGES, http://linkedlifedata.com/resource/pubmed/keyword/MICROSCOPY, ELECTRON, http://linkedlifedata.com/resource/pubmed/keyword/MURAMIDASE, http://linkedlifedata.com/resource/pubmed/keyword/MYCOBACTERIUM BOVIS, http://linkedlifedata.com/resource/pubmed/keyword/PEPTIDE PEPTIDOHYDROLASES, http://linkedlifedata.com/resource/pubmed/keyword/PHAGOCYTOSIS, http://linkedlifedata.com/resource/pubmed/keyword/PROTEIN METABOLISM, http://linkedlifedata.com/resource/pubmed/keyword/RIBONUCLEASE, http://linkedlifedata.com/resource/pubmed/keyword/STAPHYLOCOCCUS
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
118
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1009-20
pubmed:dateRevised
2010-6-22
pubmed:meshHeading
pubmed:year
1963
pubmed:articleTitle
THE PARTICULATE HYDROLASES OF MACROPHAGES. II. BIOCHEMICAL AND MORPHOLOGICAL RESPONSE TO PARTICLE INGESTION.
pubmed:publicationType
Journal Article