Linked Life Data

14100971

Source:http://linkedlifedata.com/resource/pubmed/id/14100971

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1964-5-1
pubmed:abstractText
Experiments were directed toward estimation of the magnitude of error incurred by the presumption of idealized osmometric behavior in the author's recent studies of monosaccharide transport through the human erythrocyte membrane. Thick suspensions of washed cells in isotonic buffered balanced salt medium were mixed in fixed proportions with varying dilutions of a concentrate of either (a) the mixed chlorides of the medium, or (b) glucose in the isotonic medium, and the resultant freezing point and hematocrit values determined. The form of the responses in the tonicity and the cell volume, as functions of the variable dilution of sugar or salts, conformed consistently with relations derived from the classical van't Hoff-Boyle-Mariotte pressure-volume relation. However, the effective cell water contents appeared substantially less than the weight lost in conventional drying, and varied somewhat according to the index used: expressed as grams of H(2)O per milliliter of cells at isotonic volume, the cell water implied by the hematocrit behavior was 0.614 +/- 0.015 (SD); by the salt tonicity response, 0.565 +/- 0.027; by the immediate glucose tonicity response, 0.562 +/- 0.044; and by the equilibrium glucose tonicities, 0.589 +/- 0.043. Olmstead's reports of gross deviation from the van't Hoff relation, in the rabbit red cell's responses to tonicity displacement, are attributed primarily to a systematic aberration in his method of data analysis, the observations themselves agreeing substantially with the present findings.
pubmed:commentsCorrections
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1295
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
585-603
pubmed:dateRevised
2010-6-22
pubmed:meshHeading
pubmed:year
1964
pubmed:articleTitle
THE OSMOTICALLY FUNCTIONAL WATER CONTENT OF THE HUMAN ERYTHROCYTE.
pubmed:publicationType
Journal Article