Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
1992-11-10
pubmed:abstractText
We have shown that platelets are capable of phagocytosing liposomes rather than simply sequestering particles as previously postulated. Incubation of human platelets with small neutral unilamellar liposomes (approximately 74 nm) resulted in uptake of the liposomes and retention of the lipid with rapid release of the aqueous-phase components. The lipid label [3H]-cholesterylhexadecyl ether and water-soluble [3H]inulin were used to study the fate of the liposome components. Uptake of liposomes was proportional to the number of liposomes added and to the incubation time. Approximately 250 liposomes per platelet were taken up within a 5-hr incubation period. Uptake of the liposomes occurred through the open-channel system, as evidenced by thin-section electron microscopy, and was followed by accumulation and degradation in acid- and esterase-containing vesicles, as determined by changes in fluorescence of the pH-sensitive probe, pyranine (1-hydroxypyrene-3,6,8-trisulfonic acid), and hydrolysis of the cholesteryl [14C]oleate membrane marker. Uptake was inhibited by the addition of EDTA, cytochalasin B, or 2,4-dinitrophenol and iodoacetate to the medium. Results from the serotonin release assay, micro-aggregation assay, fluorescein diacetate membrane integrity assay, and electron microscopy indicate that neither the conditions for loading nor phagocytosis of liposomes significantly alter platelet function or morphology.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-13699116, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-13909544, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-2162207, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-2654560, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-2844282, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-3651512, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-4127699, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-4159017, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-4357633, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-4632008, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-4736475, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-4957257, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-4960958, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-5086899, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-5521660, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-560940, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-56961, http://linkedlifedata.com/resource/pubmed/commentcorrection/1409624-603028
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9191-5
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Phagocytosis of liposomes by human platelets.
pubmed:affiliation
California Institute of Technology, Division of Chemistry and Chemical Engineering, Pasadena 91125.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't