| pubmed-article:1409607 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1409607 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:1409607 | lifeskim:mentions | umls-concept:C0162326 | lld:lifeskim |
| pubmed-article:1409607 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:1409607 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:1409607 | lifeskim:mentions | umls-concept:C1707689 | lld:lifeskim |
| pubmed-article:1409607 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:1409607 | pubmed:dateCreated | 1992-11-10 | lld:pubmed |
| pubmed-article:1409607 | pubmed:abstractText | We present a general strategy for designing proteins to recognize DNA sequences and illustrate this with an example based on the "Y-shaped scissors grip" model for leucine-zipper gene-regulatory proteins. The designed protein is formed from two copies, in tandem, of the basic (DNA binding) region of v-Jun. These copies are coupled through a tripeptide to yield a "dimer" expected to recognize the sequence TCATCGATGA (the v-Jun-v-Jun homodimer recognizes ATGACTCAT). We synthesized the protein and oligonucleotides containing the proposed binding sites and used gel-retardation assays and DNase I footprinting to establish that the dimer binds specifically to the DNA sequence TCATCGATGA but does not bind to the wild-type DNA sequences, nor to oligonucleotides in which the recognition half-site is modified by single-base changes. These results also provide strong support for the Y-shaped scissors grip model for binding of leucine-zipper proteins. | lld:pubmed |
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| pubmed-article:1409607 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1409607 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1409607 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1409607 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1409607 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1409607 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:1409607 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:1409607 | pubmed:author | pubmed-author:GoddardW... | lld:pubmed |
| pubmed-article:1409607 | pubmed:author | pubmed-author:CampbellJ LJL | lld:pubmed |
| pubmed-article:1409607 | pubmed:author | pubmed-author:ParkCC | lld:pubmed |
| pubmed-article:1409607 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1409607 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:1409607 | pubmed:volume | 89 | lld:pubmed |
| pubmed-article:1409607 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1409607 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1409607 | pubmed:pagination | 9094-6 | lld:pubmed |
| pubmed-article:1409607 | pubmed:dateRevised | 2010-9-7 | lld:pubmed |
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| pubmed-article:1409607 | pubmed:meshHeading | pubmed-meshheading:1409607-... | lld:pubmed |
| pubmed-article:1409607 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1409607 | pubmed:articleTitle | Protein stitchery: design of a protein for selective binding to a specific DNA sequence. | lld:pubmed |
| pubmed-article:1409607 | pubmed:affiliation | Materials and Molecular Simulation Center, Beckman Institute (139-74), Pasadena, CA. | lld:pubmed |
| pubmed-article:1409607 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1409607 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:1409607 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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