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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1992-11-10
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pubmed:abstractText |
We present a general strategy for designing proteins to recognize DNA sequences and illustrate this with an example based on the "Y-shaped scissors grip" model for leucine-zipper gene-regulatory proteins. The designed protein is formed from two copies, in tandem, of the basic (DNA binding) region of v-Jun. These copies are coupled through a tripeptide to yield a "dimer" expected to recognize the sequence TCATCGATGA (the v-Jun-v-Jun homodimer recognizes ATGACTCAT). We synthesized the protein and oligonucleotides containing the proposed binding sites and used gel-retardation assays and DNase I footprinting to establish that the dimer binds specifically to the DNA sequence TCATCGATGA but does not bind to the wild-type DNA sequences, nor to oligonucleotides in which the recognition half-site is modified by single-base changes. These results also provide strong support for the Y-shaped scissors grip model for binding of leucine-zipper proteins.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-1871104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-1991112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-2105492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-2111578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-2145515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-2261459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-2389142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-2389143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-2494459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-2494630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-2494701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-2511004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-2530632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-2683088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-2911757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-3040261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-3289117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1409607-4692853
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9094-6
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1409607-Amino Acid Sequence,
pubmed-meshheading:1409607-Base Sequence,
pubmed-meshheading:1409607-DNA,
pubmed-meshheading:1409607-DNA-Binding Proteins,
pubmed-meshheading:1409607-Deoxyribonuclease I,
pubmed-meshheading:1409607-Leucine Zippers,
pubmed-meshheading:1409607-Models, Molecular,
pubmed-meshheading:1409607-Molecular Sequence Data,
pubmed-meshheading:1409607-Nucleic Acid Conformation,
pubmed-meshheading:1409607-Oligodeoxyribonucleotides,
pubmed-meshheading:1409607-Oncogene Protein p65(gag-jun),
pubmed-meshheading:1409607-Protein Conformation
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pubmed:year |
1992
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pubmed:articleTitle |
Protein stitchery: design of a protein for selective binding to a specific DNA sequence.
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pubmed:affiliation |
Materials and Molecular Simulation Center, Beckman Institute (139-74), Pasadena, CA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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