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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1992-10-26
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pubmed:abstractText |
The influence of astrocytes on the development of cerebral cortical neurons was studied in a co-culture system using transwells with chemically defined medium. Cerebral cortical neurons from 15- or 16-day-old mouse embryo were cultured in the lower wells which were separated by a porous membrane from the upper transwells where cerebral cortical astrocytes from newborn mouse were cultured. Neurons co-cultured with astrocytes for 7 days formed a network-like web and maintained a slightly better survival from 4 to 7 days. However, neurons cultured in conditioned medium obtained from astrocytes did not form any network after 7 days even though they maintained a better cell survival at 4 days.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0304-3940
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
138
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
103-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1407647-Animals,
pubmed-meshheading:1407647-Astrocytes,
pubmed-meshheading:1407647-Cell Communication,
pubmed-meshheading:1407647-Cells, Cultured,
pubmed-meshheading:1407647-Cerebral Cortex,
pubmed-meshheading:1407647-Culture Techniques,
pubmed-meshheading:1407647-Embryo, Mammalian,
pubmed-meshheading:1407647-Glial Fibrillary Acidic Protein,
pubmed-meshheading:1407647-Mice,
pubmed-meshheading:1407647-Mice, Inbred Strains,
pubmed-meshheading:1407647-Neurites,
pubmed-meshheading:1407647-Neurons
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pubmed:year |
1992
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pubmed:articleTitle |
Neuronal survival and neurite extension supported by astrocytes co-cultured in transwells.
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pubmed:affiliation |
New Drug Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Osaka, Japan.
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pubmed:publicationType |
Journal Article
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