Linked Life Data

14069244

Source:http://linkedlifedata.com/resource/pubmed/id/14069244

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1964-3-1
pubmed:abstractText
Part of the RNA synthesized from nucleoside triphosphate precursors by partially purified RNA synthetase, an enzyme induced in Escherichia coli by the RNA-containing phage MS 2, is resistant to hydrolysis by ribonuclease. Upon heating in 0.15M sodium chloride, 0.015M sodium citrate followed by fast cooling the material becomes ribonuclease-sensitive with a sharp transition at 102 degrees to 104 degrees C. The suggestion that the ribonuclease-resistant product is double-stranded RNA is reinforced by restoration of the ribonuclease resistance of the heat-denatured material by reannealing at temperatures just below the transition point and by its buoyant density in cesium sulfate. It is suggested that double-stranded RNA is the replicative form of MS 2 phage RNA.
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0036-8075
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
142
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1188-91
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1963
pubmed:articleTitle
DOUBLE-STRANDED RIBONUCLEIC ACID FORMATION IN VITRO BY MS 2 PHAGE-INDUCED RNA SYNTHETASE.
pubmed:publicationType
Journal Article