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pubmed:abstractText |
Onoue, Kaoru (Kyushu University, Fukuoka, Japan), Masayasu Kitagawa, and Yuichi Yamamura. Chemical studies on cellular components of Bordetella pertussis. III. Isolation of highly potent toxin from Bordetella pertussis. J. Bacteriol. 86:648-655. 1963.-The thermolabile toxin of Bordetella pertussis was extracted at alkaline pH with 0.15 m saline from the disrupted cells. The toxin was purified successively by calcium phosphate gel treatment, ammonium sulfate fractionation, precipitation with potassium phosphate at alkaline pH, and finally by chromatography on a diethylaminoethyl cellulose column. Although the purified toxin still contained a small amount of agglutinin-absorbing activity, and inhomogeneity was detected by the agar gel diffusion test, the lethal and skin-necrotizing activities were much higher than those previously reported. The data obtained suggest that the toxin is proteinaceous.
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