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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0008266,
umls-concept:C0017262,
umls-concept:C0032520,
umls-concept:C0085177,
umls-concept:C0185117,
umls-concept:C0205396,
umls-concept:C0242968,
umls-concept:C0439064,
umls-concept:C0599894,
umls-concept:C0679058,
umls-concept:C0996931,
umls-concept:C1521840,
umls-concept:C1547699,
umls-concept:C2700640,
umls-concept:C2911684
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pubmed:issue |
3
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pubmed:dateCreated |
1992-11-23
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pubmed:databankReference |
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pubmed:abstractText |
Pre-mRNA processing in eukaryotic cells requires the participation of multiple protein factors and ribonucleoprotein particles. One class of proteins involved in this process are RNA-binding proteins, which contain a domain of ca. 90 amino acids with a characteristic ribonucleoprotein consensus sequence (RNP-CS). A PCR approach that is suitable for the characterization of RNP-CS-type proteins is described. Fifteen different RNA-binding domains were amplified from Nicotiana tabacum (tobacco) using oligonucleotide primers specific for the sequences (K/R)G(F/Y)(G/A)FVX(F/Y) and (L/I/V)(F/Y)(V/I)(G/K)(N/G)L, which are conserved in known RNP-CS proteins. Using the tobacco domains as probes, cDNAs encoding two RNA-binding proteins, each containing two RNP-CS-type domains, were characterized in N. plumbaginifolia. The proteins, designated CP-RBP30 and CP-RBP31, are targeted to chloroplasts as demonstrated by expression of epitope-tagged cDNAs in transfected protoplasts, followed by indirect immunofluorescence. High levels of mRNA for each protein were found in leaves but not in roots, and expression of the CP-RBP31 mRNA was strongly regulated by light. The N. plumbaginifolia proteins described in this work are distinct from chloroplast RNA-binding proteins characterized recently in tobacco and spinach.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0026-8925
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
234
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
390-400
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1406585-Amino Acid Sequence,
pubmed-meshheading:1406585-Base Sequence,
pubmed-meshheading:1406585-Chloroplasts,
pubmed-meshheading:1406585-Consensus Sequence,
pubmed-meshheading:1406585-DNA,
pubmed-meshheading:1406585-Fluorescent Antibody Technique,
pubmed-meshheading:1406585-Gene Expression,
pubmed-meshheading:1406585-Light,
pubmed-meshheading:1406585-Molecular Sequence Data,
pubmed-meshheading:1406585-Oligodeoxyribonucleotides,
pubmed-meshheading:1406585-Plant Proteins,
pubmed-meshheading:1406585-Plants, Toxic,
pubmed-meshheading:1406585-Polymerase Chain Reaction,
pubmed-meshheading:1406585-RNA, Messenger,
pubmed-meshheading:1406585-RNA-Binding Proteins,
pubmed-meshheading:1406585-Ribonucleoproteins,
pubmed-meshheading:1406585-Sequence Alignment,
pubmed-meshheading:1406585-Tobacco
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pubmed:year |
1992
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pubmed:articleTitle |
Multiple plant RNA binding proteins identified by PCR: expression of cDNAs encoding RNA binding proteins targeted to chloroplasts in Nicotiana plumbaginifolia.
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pubmed:affiliation |
Friedrich Miescher-Institut, Basel, Switzerland.
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pubmed:publicationType |
Journal Article,
Comparative Study
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