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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1992-10-30
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pubmed:abstractText |
Antibodies specific to the dopamine D2 receptor have been raised in rabbits using synthetic peptides. The resulting antiserum was sensitive to picogram quantities of peptide as measured by enzyme-linked immunoassay and was shown to have a 33% cross-reactivity with partially purified D2 receptor protein. No detectable cross-reactivity with similarly prepared fungal membranes was observed. D2 receptor preparations from normal rat pituitary cells were used in Western blot analysis. Bands of M(r) = 95,000 and 34,000 were detected in these preparations with a third faint band at 120,000. These correspond to the pituitary D2 receptor.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-0795
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
134
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
227-33
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:1402532-Animals,
pubmed-meshheading:1402532-Antibodies,
pubmed-meshheading:1402532-Antibody Specificity,
pubmed-meshheading:1402532-Blotting, Western,
pubmed-meshheading:1402532-Cell Membrane,
pubmed-meshheading:1402532-Cross Reactions,
pubmed-meshheading:1402532-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1402532-Female,
pubmed-meshheading:1402532-Pituitary Gland,
pubmed-meshheading:1402532-Rabbits,
pubmed-meshheading:1402532-Rats,
pubmed-meshheading:1402532-Receptors, Dopamine D2,
pubmed-meshheading:1402532-Rhizoctonia
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pubmed:year |
1992
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pubmed:articleTitle |
Preparation and characterization of an antibody specific to the rat dopamine D2 receptor.
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pubmed:affiliation |
Unit of Metabolic Medicine, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, Paddington, London, U.K.
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pubmed:publicationType |
Journal Article
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