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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1992-10-26
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pubmed:abstractText |
In the present report we have assessed the extent to which Ficoll-Paque separation and cryopreservation of mononuclear cells alter the measurement of lymphocyte subsets by flow cytometry. Standard Ficoll-Paque separation increased the percentage of CD4+, CD19+ and CD4+CD45RA+ cells, as well as decreasing that of CD8+, and CD4+CD29+ cells, compared to the fresh whole blood lysis technique. Moreover, cryopreservation caused a depletion of CD4+ p80+ cells, but normal whole blood values were restored following a short incubation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1759
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
154
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7-10
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1401946-Adult,
pubmed-meshheading:1401946-Antigens, CD,
pubmed-meshheading:1401946-Cryopreservation,
pubmed-meshheading:1401946-Female,
pubmed-meshheading:1401946-Ficoll,
pubmed-meshheading:1401946-Flow Cytometry,
pubmed-meshheading:1401946-Humans,
pubmed-meshheading:1401946-Immunophenotyping,
pubmed-meshheading:1401946-Lymphocytes,
pubmed-meshheading:1401946-Male,
pubmed-meshheading:1401946-Sensitivity and Specificity
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pubmed:year |
1992
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pubmed:articleTitle |
Lymphocyte immunophenotyping by flow cytometry in normal adults. Comparison of fresh whole blood lysis technique, Ficoll-Paque separation and cryopreservation.
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pubmed:affiliation |
Servei d'Immunologia, Hospital Duran i Reynals, Barcelona, Catalunya, Spain.
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pubmed:publicationType |
Journal Article,
Comparative Study
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