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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1992-11-10
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pubmed:abstractText |
A protein of 58,000-Da molecular mass was purified from the supernatant fluid of a dialysis sac culture of Listeria monocytogenes by cation-exchange chromatography. The purified protein, homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and possessing the characteristics of listeriolysin O (LLO), was used to develop an indirect enzyme-linked immunosorbent assay. Anti-LLO antibodies were shown to be consistently produced in sheep after experimental challenge with L. monocytogenes serovar 4b. The assay also successfully detected and measured specific anti-LLO antibodies in the sera of silage-fed sheep among which listeric enteritis and abortions had occurred.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1400971-1788482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1400971-1903746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1400971-1937824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1400971-1969016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1400971-2498153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1400971-2824384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1400971-3110067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1400971-3150978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1400971-3920813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1400971-4049730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1400971-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1400971-6172996
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2705-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:1400971-Animals,
pubmed-meshheading:1400971-Antibodies, Bacterial,
pubmed-meshheading:1400971-Bacterial Proteins,
pubmed-meshheading:1400971-Bacterial Toxins,
pubmed-meshheading:1400971-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1400971-Heat-Shock Proteins,
pubmed-meshheading:1400971-Hemolysin Proteins,
pubmed-meshheading:1400971-Listeriosis,
pubmed-meshheading:1400971-Serologic Tests,
pubmed-meshheading:1400971-Sheep,
pubmed-meshheading:1400971-Sheep Diseases
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pubmed:year |
1992
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pubmed:articleTitle |
Purification of listeriolysin O and development of an immunoassay for diagnosis of listeric infections in sheep.
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pubmed:affiliation |
Edinburgh Veterinary Investigation Centre, Scottish Agricultural College, Penicuik, Midlothian.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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