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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1992-10-26
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pubmed:abstractText |
Seventy-eight severe combined immunodeficiency (SCID) mice were administered intraperitoneally 1 x 10(7) to 9 x 10(7) human peripheral blood mononuclear cells (PBL) in five experiments. Human immunoglobulin G (IgG) was detected in 70 to 88% of these SCID-PBL-Hu mice after cell transplantation, and all four subclasses were present. The total concentration of human IgG varied from less than 1 to 10.2 g/liter. The SCID-PBL-Hu mice with high concentrations of human IgG regularly had mono- or oligoclonal human IgG bands in serum, as demonstrated by agarose gel electrophoresis. Of the SCID-PBL-Hu mice that were immunized with a 23-valent pneumococcal polysaccharide vaccine, 63 to 78% developed a significant human IgG antipneumococcal antibody response, whereas only very low levels of human IgM and no human IgA antipneumococcal antibodies could be detected. Twelve to twenty-two percent of the SCID-PBL-Hu mice showed signs of leakiness; these mice developed a significant mouse IgM antipneumococcal antibody response and no human antibodies. SCID-PBL-Hu mice were challenged intraperitoneally with 10 50% lethal doses of Streptococcus pneumoniae serotype 4 to study the protective effect of immunization with pneumococcal vaccine. The immunized SCID-PBL-Hu mice showed less bacteremia than did all control groups, and survival was 45 to 60%. None of the unimmunized SCID-PBL-Hu mice survived.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-1312475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-15336100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-1731222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-1804777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-1847355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-1893614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-1985122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-1991850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-2060583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-2319167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-2388039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-2509142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-2530058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-2584930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-2908877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-2922573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-2970594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-2971269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-3093081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-3201256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-3372993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-3425323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-3559207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-3570469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-3722865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-3899923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-4113792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-4956917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-5637478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-6711306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-6802537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-6823332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-7076292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-7252411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-7327193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1398925-96123
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4146-53
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1398925-Adult,
pubmed-meshheading:1398925-Animals,
pubmed-meshheading:1398925-Antibodies, Bacterial,
pubmed-meshheading:1398925-Bacterial Vaccines,
pubmed-meshheading:1398925-Female,
pubmed-meshheading:1398925-Humans,
pubmed-meshheading:1398925-Immunity, Innate,
pubmed-meshheading:1398925-Immunization,
pubmed-meshheading:1398925-Immunoglobulin G,
pubmed-meshheading:1398925-Leukocytes, Mononuclear,
pubmed-meshheading:1398925-Male,
pubmed-meshheading:1398925-Mice,
pubmed-meshheading:1398925-Mice, SCID,
pubmed-meshheading:1398925-Models, Biological,
pubmed-meshheading:1398925-Pneumococcal Vaccines,
pubmed-meshheading:1398925-Streptococcus pneumoniae,
pubmed-meshheading:1398925-Transplantation, Heterologous
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pubmed:year |
1992
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pubmed:articleTitle |
SCID-Hu mice immunized with a pneumococcal vaccine produce specific human antibodies and show increased resistance to infection.
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pubmed:affiliation |
Department of Immunology, National Institute of Public Health, Oslo, Norway.
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pubmed:publicationType |
Journal Article
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