Linked Life Data

139592

Source:http://linkedlifedata.com/resource/pubmed/id/139592

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1977-5-25
pubmed:abstractText
Blood from 10 normal healthy adults and cord blood from 8 healthy full term infants were infiltrated through a mixture sulfoethylethycellulose-Sephadex G 25 in order to eliminate the platelets and the leukocytes. Then the erythrocytes were fractionated into young and old cells by centrifugation in microhematocrit tubes. The enzyme activity and the immunologic reactivity of glucose phosphate isomerase (EC.5.3.1.9), phosphoglycerate kinase (EC.2.7.2.3), pyruvate kinase (ec.2.7.1.40), glucose 6-phosphate dehydrogenase (EC. 1.1.1.49), and 6-phosphogluconate dehydrogenase (EC.1.1.1.44) were measured in every fraction. As previously reported, the enzyme activities were far higher in cord blood than in adult blood red cells; nevertheless, the age-related loss of enzyme activity was similar in both cord and adult blood. The decrease of the enzyme activity of glucose phosphate isomerase and phosphoglycerate kinase in old cells was singly associated with a lowered concentration of the enzyme-related antigen; by contrast, the age-related decrease of the enzyme activity of pyruvate kinase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase was associated with both a lowered concentration of the enzyme-related antigen and a lowered "molecular specific activity" (i.e., a lowered ratio of enzyme activity to enzyme-related antigen concentration). This phenomenon was especially marked for pyruvate kinase, which had a molecular specific activity in old cells that was 68% of that in young cells. Phosphofructokinase had a lower enzyme activity in cord blood erythrocytes than in adult blood erythrocytes; the difference was especially important in old cells from infants in which phosphofructokinase activity was 53% of that in old cells from adults. Phosphofructokinase from old cells of full term infants and from unfractionated cells from two premature infants (21 and 32 weeks of gestation) was less neutralized by anti-muscle phosphofructokinase serum and more inhibited by ATP than the enzyme from adult blood erythrocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0031-3998
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
271-6
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:139592-Adenosine Triphosphate, pubmed-meshheading:139592-Adult, pubmed-meshheading:139592-Erythrocyte Aging, pubmed-meshheading:139592-Erythrocyte Count, pubmed-meshheading:139592-Erythrocytes, pubmed-meshheading:139592-Fetal Blood, pubmed-meshheading:139592-Glucose-6-Phosphate Isomerase, pubmed-meshheading:139592-Glucosephosphate Dehydrogenase, pubmed-meshheading:139592-Humans, pubmed-meshheading:139592-Immunoelectrophoresis, pubmed-meshheading:139592-Infant, Newborn, pubmed-meshheading:139592-Infant, Premature, pubmed-meshheading:139592-Phosphofructokinase-1, pubmed-meshheading:139592-Phosphogluconate Dehydrogenase, pubmed-meshheading:139592-Phosphoglycerate Kinase, pubmed-meshheading:139592-Pyruvate Kinase, pubmed-meshheading:139592-Reticulocytes, pubmed-meshheading:139592-Time Factors
pubmed:year
1977
pubmed:articleTitle
Immunologic study of the age-related loss of activity of six enzymes in the red cells from newborn infants and adults--evidence for a fetal type of erythrocyte phosphofructokinase.
pubmed:publicationType
Journal Article