rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
|
pubmed:dateCreated |
1992-11-13
|
pubmed:abstractText |
Primers were designed to amplify by PCR a 509-bp genomic fragment from male pig DNA, using the porcine male-specific repeat sequence described by McGraw et al. (1988). This PCR product showed male-specific hybridization in Southern blots. Nonradioactive in situ hybridization localized it to the entire length of the heterochromatic portion of Yq. The assignment was confirmed using the PCR primer pDYZ1-S for primed in situ labeling.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:issn |
0301-0171
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
61
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
152-4
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:1395727-Animals,
pubmed-meshheading:1395727-Base Sequence,
pubmed-meshheading:1395727-Chromosome Mapping,
pubmed-meshheading:1395727-DNA,
pubmed-meshheading:1395727-Heterochromatin,
pubmed-meshheading:1395727-Karyotyping,
pubmed-meshheading:1395727-Male,
pubmed-meshheading:1395727-Molecular Sequence Data,
pubmed-meshheading:1395727-Oligodeoxyribonucleotides,
pubmed-meshheading:1395727-Polymerase Chain Reaction,
pubmed-meshheading:1395727-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:1395727-Swine,
pubmed-meshheading:1395727-Y Chromosome
|
pubmed:year |
1992
|
pubmed:articleTitle |
Assignment of a porcine male-specific DNA repeat to Y-chromosomal heterochromatin.
|
pubmed:affiliation |
Department of Animal Science and Animal Health, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|