Linked Life Data

1391740

Source:http://linkedlifedata.com/resource/pubmed/id/1391740

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-11-18
pubmed:abstractText
The present study explores the ability of rabbit oviductal membranes to bind tritiated platelet-activating factor [3H]PAF on days 3 and 6 of pregnancy. Under optimal conditions (25 degrees C, 120 min) equilibrium saturation analysis revealed only one class of binding sites, characterized by Kd s(nM), 80.03 +/- 11.60 and 11.17 +/- 7.09 and Bmaxs, (pmol/mg protein), 5.25 +/- 2.23 and 1.08 +/- 0.22 (N = 3, mean +/- SEM) for ampullar membranes on days 3 and 6, respectively. The corresponding values for isthmic membranes were Kds, 86.56 +/- 12.01 and 52.43 +/- 30.49 and Bmaxs, 9.41 +/- 0.67 and 2.88 +/- 1.96 for days 3 and 6, respectively. Significant differences between days 3 and 6 were observed only in the binding affinities for the ampullar membranes and the binding capacities for the isthmic binding sites. [3H]PAF binding was inhibited in the following order of decreasing potency: lyso-PAF greater than PAF C18:0 greater than U66985 greater than PAF C16:0 for day 3 ampullar membranes; and lyso-PAF C16:0 greater than PAF C18:0 greater than U66985 greater than PAF C16:0 for day 6 ampullar membranes. These studies show the existence of specific oviductal membrane PAF binding sites, the binding parameters of which may be related to the stage of pregnancy, rather than to the spatial location along the oviduct. The relative proportion of endosalpinx to myosalpinx between the ampulla and isthmus may have masked inherent differences and account for the relatively low affinity binding. The physiological significance of oviductal membrane PAF binding is yet to be established.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0921-8319
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
77-96
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Binding of platelet-activating factor to oviductal membranes during early pregnancy in the rabbit.
pubmed:affiliation
Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.