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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1992-11-5
|
| pubmed:abstractText |
Cytokine secretion by endometrial cells from estrous and mated mice was measured using specific bioassays. The granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-6 (IL-6) contents of uterine intraluminal fluid were elevated greater than 20-fold and 250-fold respectively following mating, and both cytokines were synthesized in abundance in vitro by uterine cells harvested at estrus and on Day 1 of pregnancy. Synthesis was not impaired in genetically lymphocyte-deficient nude, SCID, or beige mice. To determine the cellular origin of the cytokines, a panning technique employing monoclonal antibodies against a range of leukocyte and other lineage markers was used to isolate uterine cell subsets in vitro. These experiments identified glandular and/or luminal epithelial cells as the major source of GM-CSF and IL-6 in estrous and pregnant uteri. Stromal fibroblasts also synthesized IL-6, as did macrophages in mated mice. Epithelial cells harvested from midgestation uteri secreted GM-CSF and IL-6 in quantities similar to those of cells from estrous and mated mice. Bioactivities of both cytokines derived from epithelial cells were neutralized by specific antibodies, and size-exclusion chromatography of conditioned media from uterine cells revealed peaks of GM-CSF and IL-6 bioactivity with M(r) 23,000 and 23,000-26,000, respectively. Bioassay of luminal fluids and culture supernatants were negative for the cytokines interleukin-1, interleukin-2, interleukin-3, and tumor necrosis factor-alpha. These studies identify murine uterine epithelium as a potent source of the cytokines GM-CSF and IL-6, which we postulate have potentially important functions in pregnancy through actions on target cells in both the uterus and the conceptus.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0006-3363
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
46
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1069-79
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1391304-Animals,
pubmed-meshheading:1391304-Cells, Cultured,
pubmed-meshheading:1391304-Culture Media,
pubmed-meshheading:1391304-Endometrium,
pubmed-meshheading:1391304-Epithelium,
pubmed-meshheading:1391304-Estrus,
pubmed-meshheading:1391304-Female,
pubmed-meshheading:1391304-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:1391304-Immunohistochemistry,
pubmed-meshheading:1391304-Interleukin-6,
pubmed-meshheading:1391304-Mice,
pubmed-meshheading:1391304-Mice, Inbred BALB C,
pubmed-meshheading:1391304-Mice, Inbred C57BL,
pubmed-meshheading:1391304-Mice, Inbred CBA,
pubmed-meshheading:1391304-Mice, Nude,
pubmed-meshheading:1391304-Mice, SCID,
pubmed-meshheading:1391304-Molecular Weight,
pubmed-meshheading:1391304-Pregnancy,
pubmed-meshheading:1391304-Pregnancy, Animal,
pubmed-meshheading:1391304-Uterus
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Uterine epithelial cells synthesize granulocyte-macrophage colony-stimulating factor and interleukin-6 in pregnant and nonpregnant mice.
|
| pubmed:affiliation |
Department of Obstetrics and Gynaecology, University of Adelaide, South Australia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|