Source:http://linkedlifedata.com/resource/pubmed/id/13890280
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| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1962-12-1
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| pubmed:abstractText |
Englesberg, E. (University of Pittsburgh, Pittsburgh, Pa.), R L. Anderson, R. Weinberg, N. Lee, P. Hoffee, G. Huttenhauer, and H. Boyer. l-Arabinose-sensitive, l-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli. J. Bacteriol. 84:137-146. 1962-l-Arabinose-negative mutants of Escherichia coli B/r, ara-53 and ara-139, are deficient in the enzyme l-ribulose 5-phosphate 4-epimerase; ara-53, further analyzed, accumulates large quantities of l-ribulose 5-phosphate when incubated with l-arabinose. The mutant sites are closely linked to the left of the previously ordered l-arabinose mutant sites, and probably represent the structural gene for l-ribulose 5-phosphate 4-epimerase (gene D) in the l-arabinose operon. The inducible levels of l-arabinose isomerase and l-ribulose 5-phosphate 4-epimerase vary correspondingly as a result of mutation in the structural gene for l-ribulokinase (gene B), further substantiating the dual structural and regulatory function of this gene locus. Ara-53 and ara-139 are strongly inhibited by l-arabinose and give rise to l-arabinose-resistant mutants. The one resistant mutant analyzed still lacks the 4-epimerase but is deficient in l-ribulokinase and has increased l-arabinose isomerase activity, a characteristic of a type of mutation in the B gene. It is proposed that accumulation of l-ribulose 5-phosphate is responsible for the inhibition, and that mutation to resistance will involve mutation in the A, B, C, permease, or repressor genes, thus providing a direct method for isolating these types of l-arabinose-negative mutants. Glucose prevents and cures the l-arabinose inhibition.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-13436491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-13436504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-13502415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-13539035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-13696894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-13702505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-13718526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-13820390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-13820391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-13829634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-13861292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-13861293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-14463540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-14907728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-16590576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13890280-16744823
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| pubmed:keyword | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
OM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
0021-9193
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
84
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
137-46
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| pubmed:dateRevised |
2009-11-18
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| pubmed:meshHeading | |
| pubmed:year |
1962
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| pubmed:articleTitle |
L-Arabinose-sensitive, L-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli.
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| pubmed:publicationType |
Journal Article
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