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rdf:type |
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lifeskim:mentions |
umls-concept:C0001758,
umls-concept:C0006556,
umls-concept:C0007600,
umls-concept:C0009015,
umls-concept:C0017337,
umls-concept:C0040690,
umls-concept:C0086418,
umls-concept:C0162801,
umls-concept:C0205263,
umls-concept:C0205314,
umls-concept:C0330390,
umls-concept:C0679622,
umls-concept:C1510779
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pubmed:issue |
7
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pubmed:dateCreated |
1992-10-26
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pubmed:databankReference |
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pubmed:abstractText |
Transforming growth factor-beta (TGF-beta) is capable of affecting the proliferation of many cell types. To identify novel genes whose protein products may mediate cellular responses to this factor, a cDNA library was made from mRNA isolated from a human lung adenocarcinoma cell line (A549) that had been treated for 3 days with TGF-beta. The library was screened by differential hybridization and a cDNA clone, beta ig-h3, was isolated. This gene was induced up to 20-fold in A549 cells after 2 days of treatment with TGF-beta 1. It was also induced in several other cell lines, including PC-3 and H2981. DNA sequence analysis of beta ig-h3 indicated that it encoded a novel protein, beta IG-H3, of 683 amino acids, which contained an amino-terminal secretory sequence and a carboxy-terminal Arg-Gly-Asp (RGD) sequence that can serve as a ligand recognition site for several integrins. beta IG-H3 also contained short amino acid regions homologous to similar regions in Drosophila fasciclin-I and four homologous internal domains, which can be folded into a potential bivalent structure and could act as a bridge between cells expressing the appropriate ligand. beta ig-h3 RNA was detected in several cell lines and tissues. COS cells transfected with plasmids encoding beta IG-H3 secreted a major 68-kD protein that was detected by immunoblotting using antipeptide antibodies. Since beta ig-h3 is induced in several cell lines whose proliferation is affected by TGF-beta 1, it may be involved in mediating some of the signals of this multifunctional growth modulator.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1044-5498
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
511-22
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:1388724-Adenocarcinoma,
pubmed-meshheading:1388724-Amino Acid Sequence,
pubmed-meshheading:1388724-Base Sequence,
pubmed-meshheading:1388724-Cell Differentiation,
pubmed-meshheading:1388724-Cell Division,
pubmed-meshheading:1388724-Cell Line,
pubmed-meshheading:1388724-Cloning, Molecular,
pubmed-meshheading:1388724-DNA,
pubmed-meshheading:1388724-Extracellular Matrix Proteins,
pubmed-meshheading:1388724-Humans,
pubmed-meshheading:1388724-Immunoblotting,
pubmed-meshheading:1388724-Kinetics,
pubmed-meshheading:1388724-Molecular Sequence Data,
pubmed-meshheading:1388724-Neoplasm Proteins,
pubmed-meshheading:1388724-Proteins,
pubmed-meshheading:1388724-Sequence Homology,
pubmed-meshheading:1388724-Transcription, Genetic,
pubmed-meshheading:1388724-Transfection,
pubmed-meshheading:1388724-Transforming Growth Factor beta,
pubmed-meshheading:1388724-Tumor Cells, Cultured
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pubmed:year |
1992
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pubmed:articleTitle |
cDNA cloning and sequence analysis of beta ig-h3, a novel gene induced in a human adenocarcinoma cell line after treatment with transforming growth factor-beta.
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pubmed:affiliation |
Bristol Myers Squibb, Seattle, WA 98121.
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pubmed:publicationType |
Journal Article
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