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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1992-10-7
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pubmed:abstractText |
A high-performance liquid chromatographic method for the determination of usnic acid in human plasma using diclofenac sodium as internal standard is described. Plasma proteins were precipitated with methanol. A 250 mm x 4 mm I.D. Nucleosil. C18 (5 microns) column with a mobile phase consisting of methanol-phosphate buffer (pH 7.4) (70:30, v/v) was used. Chromatography was performed at ambient temperature with flow-rate of 1 ml min-1 and ultraviolet detection at 280 nm. Each analysis required no longer than 7 min. Quantification was achieved by measurement of the peak-height ratio and the absolute recovery varied from 93.8 to 97.3%. The limit of quantitation of usnic acid in plasma was 0.25 micrograms ml-1. The intra-day relative standard deviation (R.S.D.) ranged from 1.24 to 4.53% and the inter-day R.S.D. from 2.23 to 8.25% at three different concentrations. The method was applied to the determination of plasma levels of usnic acid after intravenous and oral administration to study its disposition in a healthy male rabbit.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9673
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
575
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
167-70
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1387649-Administration, Oral,
pubmed-meshheading:1387649-Animals,
pubmed-meshheading:1387649-Benzofurans,
pubmed-meshheading:1387649-Chromatography, High Pressure Liquid,
pubmed-meshheading:1387649-Humans,
pubmed-meshheading:1387649-Injections, Intravenous,
pubmed-meshheading:1387649-Male,
pubmed-meshheading:1387649-Rabbits,
pubmed-meshheading:1387649-Temperature,
pubmed-meshheading:1387649-Ultraviolet Rays
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pubmed:year |
1992
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pubmed:articleTitle |
High-performance liquid chromatographic determination of usnic acid in plasma.
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pubmed:affiliation |
University College of Pharmaceutical Sciences, Kakatiya University, Warangal, India.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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