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rdf:type |
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lifeskim:mentions |
umls-concept:C0014346,
umls-concept:C0018850,
umls-concept:C0024264,
umls-concept:C0039097,
umls-concept:C0086418,
umls-concept:C0181586,
umls-concept:C0439097,
umls-concept:C0806987,
umls-concept:C1547348,
umls-concept:C1548795,
umls-concept:C1705241,
umls-concept:C1948023
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pubmed:issue |
3
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pubmed:dateCreated |
1992-10-7
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pubmed:abstractText |
T lymphocyte responses to heterologous or self 65-kD heat shock protein (hsp) have been implicated in the pathogenesis of various forms of arthritis. To delineate the relationship of 65-kD hsp to different synovial fluid (SF) T cell subsets, we stimulated synovial fluid (SFMC) and peripheral blood mononuclear cells (PBMC) from patients with different inflammatory rheumatic diseases and from healthy controls with human or mycobacterial 65-kD hsp, tetanus toxoid (TT), heat-killed or live Yersinia enterocolitica. Phenotyping of the resulting T cell lines revealed an increase of up to 97% TCR-gamma delta+ lymphocytes in the 65-kD hsp-stimulated SF-derived lines. This expansion of TCR-gamma delta+ cells was less pronounced with cultures of PBMC. A preferential expansion of TCR-gamma delta+ cells was also shown after SFMC stimulation with live, but not with heat-killed Yersinia or with TT. We conclude that a common mechanism is involved in the selective expansion of TCR-gamma delta+ lymphocytes upon SFMC infection with live Yersinia or upon contact with 65-kD hsp. Out of a panel of TCR-gamma delta+ T lymphocyte clones (TLC) derived from a human 65-kD hsp-stimulated line, only a minority of TLC proliferated weakly upon restimulation with this antigen in the presence of autologous monocytes, whereas TCR-alpha beta+ TLC responded vigorously to the human 65-kD hsp and in some cases also cross-recognized the mycobacterial hsp homologue and/or heat-killed Yersinia. This implies that additional factors or cells may be present in the milieu of SFMC cultures that propagate the expansion of TCR-gamma delta+ cells in response to 65-kD hsp or live bacteria.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-1674762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-1689764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-1695022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-1899430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-1909642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-1970672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2106682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2137854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2145360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2145851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2148638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2148640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2165196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2192247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2192435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2263227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2448638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2456364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2457140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2461518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2473405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2524009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2526644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2564416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2568584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2677142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2785131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2885839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2968521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-2978114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-3553003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-3755221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1387595-6406607
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
427-33
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1387595-Cell Line,
pubmed-meshheading:1387595-Clone Cells,
pubmed-meshheading:1387595-Enterobacteriaceae,
pubmed-meshheading:1387595-Heat-Shock Proteins,
pubmed-meshheading:1387595-Humans,
pubmed-meshheading:1387595-Lymphocyte Activation,
pubmed-meshheading:1387595-Monocytes,
pubmed-meshheading:1387595-Phenotype,
pubmed-meshheading:1387595-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:1387595-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:1387595-Synovial Fluid,
pubmed-meshheading:1387595-T-Lymphocytes
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pubmed:year |
1992
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pubmed:articleTitle |
Stimulation of synovial fluid mononuclear cells with the human 65-kD heat shock protein or with live enterobacteria leads to preferential expansion of TCR-gamma delta+ lymphocytes.
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pubmed:affiliation |
First Department of Medicine, Johannes Gutenberg University, Mainz, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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