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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1992-9-3
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pubmed:abstractText |
We have studied the effects of phosphate starvation on the levels and distributions of activities of acid phosphatase and beta-hexosaminidase in cultures of Tetrahymena thermophila. The cells were grown in synthetic nutrient medium and refed every day with fresh medium. After 4 days of growth in the complete medium, the cultures were divided into two portions. One received complete medium and the other phosphate-free, but otherwise complete, medium. Population densities and activities of acid phosphatase and beta-hexosaminidase in cells plus medium and in cell-free samples were determined in aliquots removed every day before medium replacement. In cultures having complete medium the enzyme levels remained fairly constant; in the phosphate-starved cultures both total and extracellular activities of acid phosphatase increased sixfold. beta-Hexosaminidase levels remained essentially unaltered in both cases. These results indicate that phosphate starvation can induce differential increase in acid phosphatase activity in cultures of Tetrahymena. Somewhat less than 50% of the total activities of both enzymes are found in the cell-free extracellular fluid at any time.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0014-4827
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
201
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
522-5
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1386323-Acid Phosphatase,
pubmed-meshheading:1386323-Animals,
pubmed-meshheading:1386323-Culture Media,
pubmed-meshheading:1386323-Enzyme Induction,
pubmed-meshheading:1386323-Phosphates,
pubmed-meshheading:1386323-Tetrahymena thermophila,
pubmed-meshheading:1386323-beta-N-Acetylhexosaminidases
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pubmed:year |
1992
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pubmed:articleTitle |
Differential increase in activity of acid phosphatase induced by phosphate starvation in Tetrahymena.
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pubmed:affiliation |
Department of Anatomy and Cytology, Odense University, Denmark.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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