Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1977-4-15
pubmed:abstractText
Intact microsomes isolated from rat liver showed no hexose-6-phosphate dehydrogenase activity, but the enzyme was activated by Triton X-100, deoxycholate, NH4OH, glycine/NaOH, lysophosphatidylcholine, phospholipases A and C, pancreatic lipase and cholesterol esterase, and also by sonic treatment. The enzyme activation by deoxycholate, NH4OH and sonic treatments was solely due to solubilization, while that by phospholipase A appeared to be due to the detergent action of the hydrolysis products. On the other hand, the primary effects of phospholipase C, cholesterol esterase and pancreatic lipase might be accounted for by the partial removal of membrane lipids. The results of washing and trypsin digestion experiments suggested that hexose-6-phosphate dehydrogenase is one of the most firmly bound enzymes among the microsomal proteins. The catalytic properties were the same in the solubilized and the membrane-bound, activated enzymes. Feeding the rats on a high carbohydrate diet altered the extent of enzyme activation by sonication and phospholipase C treatment, suggesting that the microsomal membrane would actually undergo changes in the conformation and/or chemical composition under certain circumstances.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
496
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-11
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:13859-Alcohol Oxidoreductases, pubmed-meshheading:13859-Alkalies, pubmed-meshheading:13859-Animals, pubmed-meshheading:13859-Carboxylic Ester Hydrolases, pubmed-meshheading:13859-Cell-Free System, pubmed-meshheading:13859-Detergents, pubmed-meshheading:13859-Dietary Carbohydrates, pubmed-meshheading:13859-Enzyme Activation, pubmed-meshheading:13859-Glucosephosphate Dehydrogenase, pubmed-meshheading:13859-Hexosephosphates, pubmed-meshheading:13859-Hydrogen-Ion Concentration, pubmed-meshheading:13859-Kinetics, pubmed-meshheading:13859-Lipids, pubmed-meshheading:13859-Male, pubmed-meshheading:13859-Microsomes, Liver, pubmed-meshheading:13859-NADP, pubmed-meshheading:13859-Phenobarbital, pubmed-meshheading:13859-Rats, pubmed-meshheading:13859-Solubility, pubmed-meshheading:13859-Sonication
pubmed:year
1977
pubmed:articleTitle
Latency of microsomal hexose-6-phosphate dehydrogenase activity.
pubmed:publicationType
Journal Article