Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9-10
|
| pubmed:dateCreated |
1992-12-15
|
| pubmed:abstractText |
Scleral fibroblasts, perichondrial cells of the scleral layer of the 12-day chick embryo, always manifest a fibroblastic morphology in monolayer culture. In soft-agar culture, these cells produce two types of colonies. One type of colony, F-type, consists of adherent fibroblastic cells, and the other, C-type, is composed of scattered round chondrocytic cells. Cells of the C-type colony are surrounded by a halo of extracellular matrix, positive with Alcian blue and with an antibody to cartilage-specific proteoglycan. When a single fibroblast clone in monolayer, derived from a single scleral fibroblast, is subcultured into soft agar, the cells give rise to both C-type and F-type colonies. Further, it was found that cells constituting F-type colonies eventually separate and become spherical, and the F-type colony converts to a C-type colony (C-type conversion). In regard to the C-type convertibility, the primary fibroblast clones were divided into four categories, early time differentiating, middle-time differentiating, late-time differentiating and nondifferentiating. This suggests that the scleral perichondrial layer of the 12-day chick embryo is composed of a variety of cells with different chondrogenic potentialities maintained in each individual cell.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0883-8364
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
28A
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
603-8
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1385381-Agar,
pubmed-meshheading:1385381-Alcian Blue,
pubmed-meshheading:1385381-Animals,
pubmed-meshheading:1385381-Cartilage,
pubmed-meshheading:1385381-Cell Differentiation,
pubmed-meshheading:1385381-Cells, Cultured,
pubmed-meshheading:1385381-Chick Embryo,
pubmed-meshheading:1385381-Clone Cells,
pubmed-meshheading:1385381-Fibroblasts,
pubmed-meshheading:1385381-Immunohistochemistry,
pubmed-meshheading:1385381-Sclera,
pubmed-meshheading:1385381-Staining and Labeling,
pubmed-meshheading:1385381-Tolonium Chloride
|
| pubmed:articleTitle |
Scleral fibroblasts of the chick embryo differentiate into chondrocytes in soft-agar culture.
|
| pubmed:affiliation |
Cell and Developmental Biology Laboratory, Faculty of Integrated Arts and Sciences, Hiroshima University, Japan.
|
| pubmed:publicationType |
Journal Article
|