Linked Life Data

1384549

Source:http://linkedlifedata.com/resource/pubmed/id/1384549

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1992-12-7
pubmed:abstractText
The inter-alpha-inhibitor (I alpha I) family encompasses four plasma proteins, namely free bikunin as well as I alpha I, pre-alpha-inhibitor (P alpha I) and inter-alpha-like inhibitor (I alpha LI). Each of the last three proteins is a distinct assembly of one bikunin chain with one or two out of three distinct, heavy (H) chains designated H1, H2 and H3. I alpha I is made of [H1 + H2 + bikunin], P alpha I is made of [H3 + bikunin] and I alpha LI is made of [H2 + bikunin]. We characterized various I alpha I family-related antisera/antibodies, including a reagent recognizing bikunin, another directed against the four H1, H2, H3 and bikunin chains, and a third one directed against both H1 and H2 chains. By a simplified absorption procedure of the latter two antisera onto solid-phase immunoadsorbents made with i) purified I alpha I, or ii) a recombinant H1 polypeptide, we also obtained specific anti-H3 or anti-H2 antibodies, respectively. This series of new antibodies was used to identify each component within the pattern of bands corresponding to I alpha I family in human serum, as separated by SDS-PAGE and western blotting.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0177-3593
pubmed:author
pubmed:issnType
Print
pubmed:volume
373
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1019-24
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Electrophoretic pattern of the inter-alpha-inhibitor family proteins in human serum, characterized by chain-specific antibodies.
pubmed:affiliation
Institut National de la Santé et de la Recherche Médicale Unit-78, Boisguillaume, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't