| pubmed-article:1384092 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1384092 | lifeskim:mentions | umls-concept:C0079459 | lld:lifeskim |
| pubmed-article:1384092 | lifeskim:mentions | umls-concept:C0080090 | lld:lifeskim |
| pubmed-article:1384092 | lifeskim:mentions | umls-concept:C0018956 | lld:lifeskim |
| pubmed-article:1384092 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:1384092 | lifeskim:mentions | umls-concept:C1332792 | lld:lifeskim |
| pubmed-article:1384092 | lifeskim:mentions | umls-concept:C0150312 | lld:lifeskim |
| pubmed-article:1384092 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:1384092 | pubmed:dateCreated | 1992-11-5 | lld:pubmed |
| pubmed-article:1384092 | pubmed:abstractText | Granulocyte colony-stimulating factor (G-CSF) was linked to NHS-biotin to yield biotinylated G-CSF (b-G-CSF), which retained the ability to stimulate colony formation by normal bone marrow (BM) cells in methylcellulose. The use of streptavidin-phycoerythrin conjugate in conjunction with flow cytometry demonstrated that the binding of biotinylated G-CSF to its receptor is saturable, competitive, and specific. A 100-fold molar excess of unlabeled G-CSF almost completely inhibited the binding of the biotinylated G-CSF to the human leukemia cell line U937, which is known to possess the G-CSF receptor. G-CSF receptors were clearly detected by flow cytometry on adult human peripheral granulocytes and monocytes, but not on lymphocytes. Using this method, the expression of G-CSF receptors on hematopoietic progenitor cells in bone marrow and umbilical cord blood, detected as CD34-positive (CD34+) cells, were examined. A small but significant number of CD34+ cells were detected among the bone marrow mononuclear cells and umbilical-cord-blood mononuclear cells (4.28% +/- 0.31%, 1.09% +/- 0.20%, respectively). The percentage of CD34+ BM mononuclear cells was significantly higher than for cord blood mononuclear cells (P less than 0.01). These CD34+ cells were then analyzed by biotinylated G-CSF binding. CD34+ cells from bone marrow contained 25.8% +/- 7.9% G-CSF receptor positive cells and those from cord blood possessed 29.2% +/- 7.0% of G-CSF receptor-positive cells. The difference was not statistically significant. | lld:pubmed |
| pubmed-article:1384092 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1384092 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1384092 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1384092 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1384092 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1384092 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1384092 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1384092 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1384092 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1384092 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1384092 | pubmed:issn | 0300-9130 | lld:pubmed |
| pubmed-article:1384092 | pubmed:author | pubmed-author:LaraF YFY | lld:pubmed |
| pubmed-article:1384092 | pubmed:author | pubmed-author:OkamuraSS | lld:pubmed |
| pubmed-article:1384092 | pubmed:author | pubmed-author:HaradaNN | lld:pubmed |
| pubmed-article:1384092 | pubmed:author | pubmed-author:ShimodaKK | lld:pubmed |
| pubmed-article:1384092 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1384092 | pubmed:volume | 192 | lld:pubmed |
| pubmed-article:1384092 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1384092 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1384092 | pubmed:pagination | 245-55 | lld:pubmed |
| pubmed-article:1384092 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:1384092 | pubmed:meshHeading | pubmed-meshheading:1384092-... | lld:pubmed |
| pubmed-article:1384092 | pubmed:meshHeading | pubmed-meshheading:1384092-... | lld:pubmed |
| pubmed-article:1384092 | pubmed:meshHeading | pubmed-meshheading:1384092-... | lld:pubmed |
| pubmed-article:1384092 | pubmed:meshHeading | pubmed-meshheading:1384092-... | lld:pubmed |
| pubmed-article:1384092 | pubmed:meshHeading | pubmed-meshheading:1384092-... | lld:pubmed |
| pubmed-article:1384092 | pubmed:meshHeading | pubmed-meshheading:1384092-... | lld:pubmed |
| pubmed-article:1384092 | pubmed:meshHeading | pubmed-meshheading:1384092-... | lld:pubmed |
| pubmed-article:1384092 | pubmed:meshHeading | pubmed-meshheading:1384092-... | lld:pubmed |
| pubmed-article:1384092 | pubmed:meshHeading | pubmed-meshheading:1384092-... | lld:pubmed |
| pubmed-article:1384092 | pubmed:meshHeading | pubmed-meshheading:1384092-... | lld:pubmed |
| pubmed-article:1384092 | pubmed:meshHeading | pubmed-meshheading:1384092-... | lld:pubmed |
| pubmed-article:1384092 | pubmed:meshHeading | pubmed-meshheading:1384092-... | lld:pubmed |
| pubmed-article:1384092 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1384092 | pubmed:articleTitle | Detection of the granulocyte colony-stimulating factor receptor using biotinylated granulocyte colony-stimulating factor: presence of granulocyte colony-stimulating factor receptor on CD34-positive hematopoietic progenitor cells. | lld:pubmed |
| pubmed-article:1384092 | pubmed:affiliation | First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan. | lld:pubmed |
| pubmed-article:1384092 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1384092 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1384092 | lld:pubmed |
