Linked Life Data

1382837

Source:http://linkedlifedata.com/resource/pubmed/id/1382837

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
1992-10-29
pubmed:abstractText
We have previously observed that breast cancer cell lines could exhibit either epithelial or fibroblastic phenotypes as reflected by their morphologies and intermediate filament protein expression (C. L. Sommers, D. Walker-Jones, S. E. Heckford, P. Worland, E. Valverius, R. Clark, M. Stampfer, and E. P. Gelmann, Cancer Res., 49:4258-4263, 1989). Fibroblastoid, vimentin-expressing breast cancer cell lines are more invasive in vitro and in vivo (E. W. Thompson, S. Paik, N. Brunner, C. L. Sommers, G. Zugmaier, R. Clarke, T. B. Shima, J. Torri, S. Donahue, M. E. Lippman, G. R. Martin, and R. B. Dickson, J. Cell. Physiol., 150: 534-544, 1992). We hypothesized that a breast cancer cell with an epithelial phenotype could undergo a transition to a fibroblastic phenotype, possibly resulting in more invasive capacity. We now show that two Adriamycin-resistant MCF-7 cell lines and a vinblastine-resistant ZR-75-B cell line have undergone such a transition. Adriamycin-resistant MCF-7 cells express vimentin, have diminished keratin 19 expression, have lost cell adhesion molecule uvomorulin expression, and have reduced formation of desmosomes and tight junctions as determined by reduced immunodetection of their components desmoplakins I and II and zonula occludens (ZO)-1. Other MCF-7 cell lines selected for resistance to vinblastine and to Adriamycin and verapamil did not have these characteristics, indicating that drug selection does not invariably cause these phenotypic changes. In addition, to determine if vimentin expression in MCF-7 cells alone could manifest a fibroblastic phenotype, we transfected the full-length human vimentin complementary DNA into MCF-7 cells. Although vimentin expression was achieved in MCF-7 cells, it did not affect the phenotype of the cells in terms of the distribution of keratins, desmoplakins I and II, ZO-1, or uvomorulin or in terms of in vitro invasiveness. We conclude that vimentin expression is a marker for a fibroblastic and invasive phenotype in breast cancer cells but does not by itself give rise to this phenotype.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5190-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1382837-Antibodies, pubmed-meshheading:1382837-Breast Neoplasms, pubmed-meshheading:1382837-Cell Adhesion Molecules, pubmed-meshheading:1382837-Cytoskeletal Proteins, pubmed-meshheading:1382837-Desmoplakins, pubmed-meshheading:1382837-Doxorubicin, pubmed-meshheading:1382837-Drug Resistance, pubmed-meshheading:1382837-Epithelium, pubmed-meshheading:1382837-Fibroblasts, pubmed-meshheading:1382837-Humans, pubmed-meshheading:1382837-Intercellular Junctions, pubmed-meshheading:1382837-Intermediate Filaments, pubmed-meshheading:1382837-Keratins, pubmed-meshheading:1382837-Neoplasm Invasiveness, pubmed-meshheading:1382837-Phenotype, pubmed-meshheading:1382837-Staining and Labeling, pubmed-meshheading:1382837-Transfection, pubmed-meshheading:1382837-Tumor Cells, Cultured, pubmed-meshheading:1382837-Tumor Markers, Biological, pubmed-meshheading:1382837-Verapamil, pubmed-meshheading:1382837-Vimentin, pubmed-meshheading:1382837-Vinblastine
pubmed:year
1992
pubmed:articleTitle
Loss of epithelial markers and acquisition of vimentin expression in adriamycin- and vinblastine-resistant human breast cancer cell lines.
pubmed:affiliation
Lombardi Cancer Research Center, Georgetown University, Washington, DC 20007.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't