Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1992-10-29
|
| pubmed:abstractText |
Human kit ligand (KL), also known as stem cell factor (SCF), steel factor, or mast cell growth factor, is a recently identified hematopoietic growth factor whose receptor is the product of the c-kit proto-oncogene. Alternative splicing of the pre-mRNA of KL/SCF results in secreted and membrane-bound forms of the protein. We and others have recently shown that the c-kit gene product is expressed on human megakaryocytes and that soluble KL/SCF in combination with granulocyte-macrophage colony-stimulating factor, interleukin-3 (IL-3), or IL-6 increased megakaryocyte progenitor colony formation (CFU-MEG) and stimulated mature megakaryocytes. Here we show that adhesion of human megakaryocytes to bone marrow stromal fibroblasts, which express the membrane-bound form of KL/SCF (mKL/SCF), is mediated in part by the interaction between mKL/SCF and the c-kit protein. This interaction also results in marrow fibroblast-stimulated proliferation but not an increase in ploidy of megakaryocytes; when the two cell types were separated by a transoluble membrane, proliferation did not occur. Adhesion and proliferation of human megakaryocytes to an immortalized murine stromal cell line SI/SI lacking the KL/SCF gene was impaired, whereas transfection of SI/SI cells with human mKL/SCF significantly increased both adhesion and proliferation. Marrow stromal fibroblast mKL/SCF may serve both as an adhesion structure and as a growth-potentiating factor for megakaryocytes in the bone marrow.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Hematopoietic Cell Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
80
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1679-84
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1382698-Animals,
pubmed-meshheading:1382698-Bone Marrow,
pubmed-meshheading:1382698-Bone Marrow Cells,
pubmed-meshheading:1382698-Cell Adhesion,
pubmed-meshheading:1382698-Cell Line,
pubmed-meshheading:1382698-Cells, Cultured,
pubmed-meshheading:1382698-DNA,
pubmed-meshheading:1382698-DNA Replication,
pubmed-meshheading:1382698-Fibroblasts,
pubmed-meshheading:1382698-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:1382698-Hematopoietic Cell Growth Factors,
pubmed-meshheading:1382698-Humans,
pubmed-meshheading:1382698-Interleukin-3,
pubmed-meshheading:1382698-Interleukin-6,
pubmed-meshheading:1382698-Megakaryocytes,
pubmed-meshheading:1382698-Mice,
pubmed-meshheading:1382698-Polymerase Chain Reaction,
pubmed-meshheading:1382698-RNA,
pubmed-meshheading:1382698-RNA, Messenger,
pubmed-meshheading:1382698-RNA Splicing,
pubmed-meshheading:1382698-Stem Cell Factor,
pubmed-meshheading:1382698-Thymidine
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Interaction of human bone marrow fibroblasts with megakaryocytes: role of the c-kit ligand.
|
| pubmed:affiliation |
Department of Medicine, New England Deaconess Hospital, Harvard Medical School, Boston, MA 02215.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|