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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1992-10-22
|
| pubmed:abstractText |
The clinical validity of a continuous colorimetric method for measuring pancreatic lipase was assessed. 1,2-Diacylglycerol containing long-chain fatty acid residues was used as substrate, and the method was adapted to a discrete analyser. The dynamic range was ascertained up to at least 30-fold the upper reference limit. Precision tests on three control sera yielded overall CVs of 4.6% (mean value 21 U/l), 2.4% (115 U/l), and 1.0% (386 U/l), respectively. Using serum samples from normal subjects and patients with pancreatic and non-pancreatic disorders, the present method was compared with a turbidimetric method (r = 0.997; n = 281) and a homogeneous enzyme immunoassay (r = 0.987; n = 93). The reference interval established on 121 healthy subjects was 8-57 U/l (central 95th percentile, median 22 U/l). The sensitivity of this lipase assay in the diagnosis of acute pancreatitis (100%, median 5.6-fold the upper reference limit) was equal to that of the pancreatic isoamylase assay, and higher than that of the total alpha-amylase assay (88.2%); the specificity for acute pancreatitis with respect to a group of patients with acute and chronic non-pancreatic abdominal diseases (91%) was higher than that of both pancreatic isoamylase (76%) and total alpha-amylase (71%).
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0939-4974
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
30
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
439-44
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1381966-Acute Disease,
pubmed-meshheading:1381966-Amylases,
pubmed-meshheading:1381966-Carcinoma,
pubmed-meshheading:1381966-Chronic Disease,
pubmed-meshheading:1381966-Clinical Enzyme Tests,
pubmed-meshheading:1381966-Colorimetry,
pubmed-meshheading:1381966-Female,
pubmed-meshheading:1381966-Humans,
pubmed-meshheading:1381966-Isoamylase,
pubmed-meshheading:1381966-Lipase,
pubmed-meshheading:1381966-Male,
pubmed-meshheading:1381966-Pancreatic Neoplasms,
pubmed-meshheading:1381966-Pancreatitis,
pubmed-meshheading:1381966-Reference Values,
pubmed-meshheading:1381966-Reproducibility of Results,
pubmed-meshheading:1381966-Sensitivity and Specificity,
pubmed-meshheading:1381966-Stomach Diseases,
pubmed-meshheading:1381966-Substrate Specificity
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Clinical validity of a continuous colorimetric method for serum lipase.
|
| pubmed:affiliation |
Servizio Analisi Chimico-Cliniche e Neurochimica, Fondazione C. Mondino, Pavia, Italia.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|