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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1992-9-4
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pubmed:abstractText |
The cleavage properties of a trans-acting hammerhead ribozyme targeted 51 bases upstream of the putative splicing branch point in the hamster prion pre-mRNA intron were investigated in cell-free model systems in vitro. The specificity of cleavage was demonstrated by the inability of this ribozyme to cleave a non-homologous synthetic message encoding part of the beta amyloid peptide precursor, beta APP, and by the inability of the prion pre-mRNA to be cleaved by a ribozyme targeted to beta amyloid peptide precursor mRNA. Also, the addition of total RNA isolated from rat brain had only a minimal effect on the cleavage of the prion substrate pre-mRNA by the ribozyme. Finally neither the presence of 100 ng of nuclear or cytoplasmic proteins were found to affect the rate of cleavage in vitro.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
186
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1171-7
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:1379801-Adenosine Triphosphate,
pubmed-meshheading:1379801-Amyloid beta-Protein Precursor,
pubmed-meshheading:1379801-Animals,
pubmed-meshheading:1379801-Base Sequence,
pubmed-meshheading:1379801-Brain,
pubmed-meshheading:1379801-Cell-Free System,
pubmed-meshheading:1379801-Cloning, Molecular,
pubmed-meshheading:1379801-Cricetinae,
pubmed-meshheading:1379801-Molecular Sequence Data,
pubmed-meshheading:1379801-Nucleic Acid Conformation,
pubmed-meshheading:1379801-Prions,
pubmed-meshheading:1379801-RNA,
pubmed-meshheading:1379801-RNA, Catalytic,
pubmed-meshheading:1379801-RNA, Messenger,
pubmed-meshheading:1379801-RNA Precursors,
pubmed-meshheading:1379801-Rats,
pubmed-meshheading:1379801-Restriction Mapping,
pubmed-meshheading:1379801-Sharks,
pubmed-meshheading:1379801-Substrate Specificity,
pubmed-meshheading:1379801-Transcription, Genetic
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pubmed:year |
1992
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pubmed:articleTitle |
Hammerhead ribozyme cleavage of hamster prion pre-mRNA in complex cell-free model systems.
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pubmed:affiliation |
New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10314.
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pubmed:publicationType |
Journal Article
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