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pubmed-article:1379790 | lifeskim:mentions | umls-concept:C0079941 | lld:lifeskim |
pubmed-article:1379790 | lifeskim:mentions | umls-concept:C1332684 | lld:lifeskim |
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pubmed-article:1379790 | lifeskim:mentions | umls-concept:C1517892 | lld:lifeskim |
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pubmed-article:1379790 | pubmed:issue | 2 | lld:pubmed |
pubmed-article:1379790 | pubmed:dateCreated | 1992-9-10 | lld:pubmed |
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pubmed-article:1379790 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1379790 | pubmed:abstractText | Restriction fragments isolated from a 17-kb rat genomic DNA clone containing the gene for apolipoprotein (apo) E were radiolabeled and used to screen a rat liver cDNA library. A cDNA clone hybridizing to a 6-kb genomic DNA fragment was isolated and the nucleotide sequence of the cDNA insert determined. The sequence was homologous to the sequence for human apo C-I and was used to derive the corresponding amino acid sequence. Unlike human apo C-I, mature rat apo C-I contains histidine, lacks valine, and has alanine at the C terminus and aspartate as the N terminus. Screening the rat liver cDNA library with a radiolabeled 1.9-kb restriction fragment from the genomic DNA clone containing the rat apo E gene identified another cDNA clone (ECL cDNA). Nucleotide sequencing yielded a derived 75-amino-acid sequence for the ECL protein with a hydrophobicity profile similar to that of rat apo C-I. Northern analysis demonstrated a 0.50-kb band for ECL mRNA. The tissue-specific expression of the gene is similar to that of rat apo C-I. This study indicates that the rat apo C-I and ECL genes are closely linked, about 4.5 and 12 kb downstream of the apo E gene, respectively. | lld:pubmed |
pubmed-article:1379790 | pubmed:language | eng | lld:pubmed |
pubmed-article:1379790 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1379790 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:1379790 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:1379790 | pubmed:month | Sep | lld:pubmed |
pubmed-article:1379790 | pubmed:issn | 0003-9861 | lld:pubmed |
pubmed-article:1379790 | pubmed:author | pubmed-author:HowlettG JGJ | lld:pubmed |
pubmed-article:1379790 | pubmed:author | pubmed-author:ShenPP | lld:pubmed |
pubmed-article:1379790 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:1379790 | pubmed:volume | 297 | lld:pubmed |
pubmed-article:1379790 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:1379790 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:1379790 | pubmed:pagination | 345-53 | lld:pubmed |
pubmed-article:1379790 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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pubmed-article:1379790 | pubmed:year | 1992 | lld:pubmed |
pubmed-article:1379790 | pubmed:articleTitle | Two coding regions closely linked to the rat apolipoprotein E gene: nucleotide sequences of rat apolipoprotein C-I and ECL cDNA. | lld:pubmed |
pubmed-article:1379790 | pubmed:affiliation | Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia. | lld:pubmed |
pubmed-article:1379790 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:1379790 | pubmed:publicationType | Comparative Study | lld:pubmed |
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