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rdf:type |
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lifeskim:mentions |
umls-concept:C0004793,
umls-concept:C0006556,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0052189,
umls-concept:C0079941,
umls-concept:C0208973,
umls-concept:C1332684,
umls-concept:C1412481,
umls-concept:C1517892,
umls-concept:C1704666
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pubmed:issue |
2
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pubmed:dateCreated |
1992-9-10
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L05509,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L05510,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L05930,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S76223,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S76225,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S76228,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S76232,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S76235,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X15512,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X56190,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X63431
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pubmed:abstractText |
Restriction fragments isolated from a 17-kb rat genomic DNA clone containing the gene for apolipoprotein (apo) E were radiolabeled and used to screen a rat liver cDNA library. A cDNA clone hybridizing to a 6-kb genomic DNA fragment was isolated and the nucleotide sequence of the cDNA insert determined. The sequence was homologous to the sequence for human apo C-I and was used to derive the corresponding amino acid sequence. Unlike human apo C-I, mature rat apo C-I contains histidine, lacks valine, and has alanine at the C terminus and aspartate as the N terminus. Screening the rat liver cDNA library with a radiolabeled 1.9-kb restriction fragment from the genomic DNA clone containing the rat apo E gene identified another cDNA clone (ECL cDNA). Nucleotide sequencing yielded a derived 75-amino-acid sequence for the ECL protein with a hydrophobicity profile similar to that of rat apo C-I. Northern analysis demonstrated a 0.50-kb band for ECL mRNA. The tissue-specific expression of the gene is similar to that of rat apo C-I. This study indicates that the rat apo C-I and ECL genes are closely linked, about 4.5 and 12 kb downstream of the apo E gene, respectively.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0003-9861
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
297
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
345-53
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:1379790-Amino Acid Sequence,
pubmed-meshheading:1379790-Animals,
pubmed-meshheading:1379790-Apolipoprotein C-I,
pubmed-meshheading:1379790-Apolipoproteins C,
pubmed-meshheading:1379790-Apolipoproteins E,
pubmed-meshheading:1379790-Base Sequence,
pubmed-meshheading:1379790-Cloning, Molecular,
pubmed-meshheading:1379790-DNA,
pubmed-meshheading:1379790-Dogs,
pubmed-meshheading:1379790-Gene Library,
pubmed-meshheading:1379790-Genetic Linkage,
pubmed-meshheading:1379790-Humans,
pubmed-meshheading:1379790-Liver,
pubmed-meshheading:1379790-Molecular Sequence Data,
pubmed-meshheading:1379790-Organ Specificity,
pubmed-meshheading:1379790-Protein Conformation,
pubmed-meshheading:1379790-RNA,
pubmed-meshheading:1379790-RNA, Messenger,
pubmed-meshheading:1379790-Rats,
pubmed-meshheading:1379790-Restriction Mapping,
pubmed-meshheading:1379790-Sequence Homology, Nucleic Acid
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pubmed:year |
1992
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pubmed:articleTitle |
Two coding regions closely linked to the rat apolipoprotein E gene: nucleotide sequences of rat apolipoprotein C-I and ECL cDNA.
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pubmed:affiliation |
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
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pubmed:publicationType |
Journal Article,
Comparative Study
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