|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
23
|
|
pubmed:dateCreated |
1992-9-10
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
Interleukin 8 (IL-8) and melanocyte growth-stimulatory activity/gro (MGSA) are structurally related proinflammatory cytokines that are chemoattractants and activators of neutrophils. Recently, cDNA clones encoding a high affinity IL-8 receptor (IL-8R-A) and a "low affinity" IL-8 receptor (IL-8R-B) have been isolated from human cDNA libraries. These two receptors have 77% amino acid identity and are members of the G protein-coupled superfamily of receptors with seven transmembrane domains. We have expressed these two receptors in mammalian cells and find that in this system both receptors bind IL-8 with high affinity (Kd approximately 2 nM). The receptor affinities differ for MGSA, however. IL-8R-A binds MGSA with low affinity (Kd approximately 450 nM); IL-8R-B binds MGSA with high affinity (Kd approximately 2 nM). The transfected cells respond to ligand binding with a transient increase in the intracellular Ca2+ concentration. A Ca2+ response is found for IL-8R-A following the binding of IL-8; no response is found for MGSA. A Ca2+ response for IL-8R-B follows the binding of both ligands. Blot hybridization with oligonucleotide probes specific for the two receptors shows that mRNA for both receptors is present in human neutrophils. Analysis of IL-8 and MGSA binding data on neutrophils as well as Ca2+ response and desensitization data shows that the presence of these two IL-8 receptors on the cell surface can account for the profile of these two ligands on neutrophils.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CXCL1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL1,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Poly A,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-8A,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
267
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
16283-7
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:1379593-Amino Acid Sequence,
pubmed-meshheading:1379593-Base Sequence,
pubmed-meshheading:1379593-Binding, Competitive,
pubmed-meshheading:1379593-Calcium,
pubmed-meshheading:1379593-Cell Line,
pubmed-meshheading:1379593-Chemokine CXCL1,
pubmed-meshheading:1379593-Chemokines, CXC,
pubmed-meshheading:1379593-Chemotactic Factors,
pubmed-meshheading:1379593-Cloning, Molecular,
pubmed-meshheading:1379593-Escherichia coli,
pubmed-meshheading:1379593-Gene Library,
pubmed-meshheading:1379593-Growth Substances,
pubmed-meshheading:1379593-Humans,
pubmed-meshheading:1379593-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:1379593-Interleukin-8,
pubmed-meshheading:1379593-Kinetics,
pubmed-meshheading:1379593-Molecular Sequence Data,
pubmed-meshheading:1379593-Molecular Weight,
pubmed-meshheading:1379593-Neutrophils,
pubmed-meshheading:1379593-Oligonucleotide Probes,
pubmed-meshheading:1379593-Poly A,
pubmed-meshheading:1379593-RNA,
pubmed-meshheading:1379593-RNA, Messenger,
pubmed-meshheading:1379593-Receptors, Immunologic,
pubmed-meshheading:1379593-Receptors, Interleukin-8A,
pubmed-meshheading:1379593-Recombinant Proteins,
pubmed-meshheading:1379593-Sequence Homology, Nucleic Acid
|
|
pubmed:year |
1992
|
|
pubmed:articleTitle |
Characterization of two high affinity human interleukin-8 receptors.
|
|
pubmed:affiliation |
Department of Molecular Biology, Genentech, Inc., South San Francisco, California 94080.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study
|
