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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0035366,
umls-concept:C0086418,
umls-concept:C0162326,
umls-concept:C0185117,
umls-concept:C0205227,
umls-concept:C0443452,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C2911684
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pubmed:issue |
8
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pubmed:dateCreated |
1992-9-10
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pubmed:databankReference |
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pubmed:abstractText |
The human salivary amylase genes are associated with two inserted elements, a gamma-actin-processed pseudogene and an endogenous retroviral-like element. To test the contribution of these inserted elements to tissue specificity, 25 lines of transgenic mice carrying 10 amylase constructs were established. A 1-kb fragment of AMY1C (-1003 to +2) was found to be sufficient for parotid-specific expression of a human growth hormone reporter gene. The 1-kb fragment is entirely derived from inserted sequences. Deletion from -1003 to -826 resulted in reduced levels of transgene expression and loss of tissue specificity. The fragment -1003 to -327 was sufficient to transfer parotid specificity to the thymidine kinase promoter. The data demonstrate that the functional tissue-specific promoter of human AMY1C is derived from inserted sequences and that parotid expression can be conferred by sequences derived solely from the retrovirus. A role for retrotransposition in the evolution of gene regulation is indicated by these and other recent observations.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0890-9369
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:geneSymbol |
AMY1C
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1457-65
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1379564-Actins,
pubmed-meshheading:1379564-Amylases,
pubmed-meshheading:1379564-Animals,
pubmed-meshheading:1379564-Base Sequence,
pubmed-meshheading:1379564-DNA Transposable Elements,
pubmed-meshheading:1379564-Gene Expression Regulation, Viral,
pubmed-meshheading:1379564-Growth Hormone,
pubmed-meshheading:1379564-Humans,
pubmed-meshheading:1379564-Mice,
pubmed-meshheading:1379564-Mice, Transgenic,
pubmed-meshheading:1379564-Molecular Sequence Data,
pubmed-meshheading:1379564-Oligodeoxyribonucleotides,
pubmed-meshheading:1379564-Parotid Gland,
pubmed-meshheading:1379564-Polymerase Chain Reaction,
pubmed-meshheading:1379564-Promoter Regions, Genetic,
pubmed-meshheading:1379564-Proviruses,
pubmed-meshheading:1379564-Radioimmunoassay,
pubmed-meshheading:1379564-Recombinant Fusion Proteins,
pubmed-meshheading:1379564-Transcription, Genetic
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pubmed:year |
1992
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pubmed:articleTitle |
Endogenous retroviral sequences are required for tissue-specific expression of a human salivary amylase gene.
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pubmed:affiliation |
Department of Human Genetics, University of Michigan, Ann Arbor 48109-0618.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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