Linked Life Data

1379394

Source:http://linkedlifedata.com/resource/pubmed/id/1379394

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-8-28
pubmed:abstractText
A second generation ELISA for combined detection of antibodies to three hepatitis C virus (HCV) recombinant proteins, i.e. C100, C33c and core, was compared with a first generation anti-HCV ELISA in which only antibodies to C100 are detected. The results of the ELISAs were evaluated in 225 haemophilia patients (panel A) and 44 patients with non-A, non-B (NANB) hepatitis (panel B). HCV infection was established by cDNA-polymerase chain reaction (PCR) (in panel B only) and by studying the anti-HCV reaction patterns in 4 separate ELISAs for detection of antibodies to the recombinant proteins C100, C33c, core and a combination of two synthetic peptides sp67/65 derived from the C100 region. The sensitivity for the detection of HCV infection had increased from 0.92[95% confidence interval (CI): 0.87-0.95] to 1.00 (95% CI: 0.89-1.00) in haemophiliacs and from 0.84 (95% CI: 0.66-0.95) to 1.00 (95% CI: 0.89-1.00) in NANB hepatitis patients when the second generation ELISA was used instead of the first generation ELISA. Concurrently the chance of a false negative result was reduced in panel A and B from 0.37 to 0 and from 0.28 to 0, respectively. Analysis of anti-HCV reaction patterns revealed that 172 of 206 (83.5%) anti-HCV ELISA-reactive haemophilia patients had antibodies to all 4 antigens tested. In the NANB hepatitis patients 18 of 31 (58.1%) anti-HCV ELISA-reactive subjects reacted with 4 antigens. In the PCR tested panel of NANB hepatitis patients 2 subjects who showed antibody reactivity to only one antigen and 5 patients with reactivity to 2 antigens were PCR-positive.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0042-9007
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
213-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1379394-Antigens, Viral, pubmed-meshheading:1379394-Blood Transfusion, pubmed-meshheading:1379394-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:1379394-False Negative Reactions, pubmed-meshheading:1379394-Hemophilia A, pubmed-meshheading:1379394-Hepacivirus, pubmed-meshheading:1379394-Hepatitis Antibodies, pubmed-meshheading:1379394-Hepatitis C, pubmed-meshheading:1379394-Hepatitis C Antigens, pubmed-meshheading:1379394-Humans, pubmed-meshheading:1379394-Male, pubmed-meshheading:1379394-Peptide Fragments, pubmed-meshheading:1379394-Polymerase Chain Reaction, pubmed-meshheading:1379394-Prevalence, pubmed-meshheading:1379394-Recombinant Proteins, pubmed-meshheading:1379394-Sensitivity and Specificity, pubmed-meshheading:1379394-Viral Core Proteins, pubmed-meshheading:1379394-Viral Nonstructural Proteins, pubmed-meshheading:1379394-Viral Proteins
pubmed:year
1992
pubmed:articleTitle
Enhanced sensitivity of a second generation ELISA for antibody to hepatitis C virus.
pubmed:affiliation
Central Laboratory of The Netherlands, Red Cross Blood Transfusion Service, Amsterdam, The Netherlands.
pubmed:publicationType
Journal Article, Comparative Study