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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
22
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pubmed:dateCreated |
1992-8-28
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pubmed:abstractText |
Regulation of transcription elongation is an important mechanism in controlling eukaryotic gene expression. SII is an RNA polymerase II-binding protein that stimulates transcription elongation and also activates nascent transcript cleavage by RNA polymerase II in elongation complexes in vitro (Reines, D. (1992) J. Biol. Chem. 267, 3795-3800). Here we show that SII-dependent in vitro transcription through an arrest site in a human gene is preceded by nascent transcript cleavage. RNA cleavage appeared to be an obligatory step in the SII activation process. Recombinant SII activated cleavage while a truncated derivative lacking polymerase binding activity did not. Cleavage was not restricted to an elongation complex arrested at this particular site, showing that nascent RNA hydrolysis is a general property of RNA polymerase II elongation complexes. These data support a model whereby SII stimulates elongation via a ribonuclease activity of the elongation complex.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15516-22
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1379232-Animals,
pubmed-meshheading:1379232-Base Sequence,
pubmed-meshheading:1379232-Humans,
pubmed-meshheading:1379232-Kinetics,
pubmed-meshheading:1379232-Liver,
pubmed-meshheading:1379232-Molecular Sequence Data,
pubmed-meshheading:1379232-Protein Binding,
pubmed-meshheading:1379232-RNA,
pubmed-meshheading:1379232-RNA Polymerase II,
pubmed-meshheading:1379232-Rats,
pubmed-meshheading:1379232-Recombinant Proteins,
pubmed-meshheading:1379232-Transcription, Genetic
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pubmed:year |
1992
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pubmed:articleTitle |
The RNA polymerase II elongation complex. Factor-dependent transcription elongation involves nascent RNA cleavage.
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pubmed:affiliation |
Graduate Program in Biochemistry and Molecular Biology, Emory University School of Medicine, Atlanta, Georgia 30322.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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