Linked Life Data

1378865

Source:http://linkedlifedata.com/resource/pubmed/id/1378865

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-8-25
pubmed:abstractText
CD40 is a prominent B cell Ag also found on certain epithelial cells and on carcinomas. In this report, we analyzed CD40 distribution in the human thymus. CD40 was not found on the majority of CD45-positive thymocytes, but was present in a CD45-negative stromal cell population. Immunohistology showed CD40 expression on cortical thymic epithelial cells (TEC), medullary TEC, thymic interdigitating cells, and thymic B cells. CD40 was not found on thymocytes, endothelial cells, or on the fibroblasts of the septae. Expression of CD40 was specifically maintained on cultured TEC and not found on cultured thymic fibroblasts. IL-1 alpha, TNF-alpha, IFN-gamma, but not IL-4, significantly up-regulated the membrane expression of CD40 on cultured TEC. The regulation of CD40 was similar to that of ICAM-1, and contrasted with that of MHC class II Ag, which could only be induced by IFN-gamma but not by IL-1, TNF, or IL-4. Northern blot analysis showed the presence of a 1.4-kb mRNA transcript for CD40 in TEC, which was up-regulated by IL-1 and IFN-gamma. Cross-linking of CD40 at the surface of human TEC in the absence of IL-1 stimulation failed to induce cytokine secretion. Triggering of TEC with anti-CD40 mAb in conjunction with IFN-gamma and IL-1 stimulation increased granulocyte-macrophage CSF secretion in a dose-dependent manner. The effect was visible as early as 24 h after triggering, occurred in the absence of cellular proliferation, and was specific for CD40 since triggering of other TEC membrane Ag such as ICAM-1 or MHC class I molecules had no effect to increase cytokine production in TEC. These data demonstrate that CD40 is expressed and is a functional molecule at the surface of the epithelial cells of the thymus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
149
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
775-82
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:1378865-Antigens, CD, pubmed-meshheading:1378865-Antigens, CD40, pubmed-meshheading:1378865-Antigens, CD45, pubmed-meshheading:1378865-Antigens, Differentiation, B-Lymphocyte, pubmed-meshheading:1378865-Cell Adhesion Molecules, pubmed-meshheading:1378865-Cytokines, pubmed-meshheading:1378865-Epithelium, pubmed-meshheading:1378865-Flow Cytometry, pubmed-meshheading:1378865-Fluorescent Antibody Technique, pubmed-meshheading:1378865-Gene Expression, pubmed-meshheading:1378865-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:1378865-HLA-D Antigens, pubmed-meshheading:1378865-Histocompatibility Antigens, pubmed-meshheading:1378865-Humans, pubmed-meshheading:1378865-Intercellular Adhesion Molecule-1, pubmed-meshheading:1378865-RNA, Messenger, pubmed-meshheading:1378865-Thymus Gland
pubmed:year
1992
pubmed:articleTitle
CD40 is functionally expressed on human thymic epithelial cells.
pubmed:affiliation
DNAX Research Institute, Department of Human Immunology, Palo Alto, CA 94304-1104.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't